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Time-Series Transcriptomic Analysis of Contrasting Rice Materials under Heat Stress Reveals a Faster Response in the Tolerant Cultivar

文献类型： 外文期刊

作者： Cai, Haiya 1 ; Wang, Hongpan 3 ; Zhou, Lei 1 ; Li, Bo 3 ; Zhang, Shuo 1 ; He, Yonggang 1 ; Guo, Ying 1 ; You, Aiqing 1 ; Jiao, Chunhai 1 ; Xu, Yanhao 1 ;

作者机构： 1.Hubei Acad Agr Sci, Food Crops Inst, Hubei Key Lab Food Crop Germplasm & Genet Improvem, Key Lab Crop Mol Breeding,Minist Agr & Rural Affai, Wuhan 430064, Peoples R China

2.Minist Agr & Rural Affairs, Sci Observat & Expt Stn Crop Gene Resources & Germ, Wuhan 430064, Peoples R China

3.Yangtze Univ, Coll Agr, Jingzhou 434025, Peoples R China

关键词： RNA-seq; dynamic response; MAPK signaling pathway; candidate gene

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN： 1661-6596

年卷期： 2023 年 24 卷 11 期

页码：

收录情况： SCI

摘要： Short-term heat stress can affect the growth of rice (Oryza sativa L.) seedlings, subsequently decreasing yields. Determining the dynamic response of rice seedlings to short-term heat stress is highly important for accelerating research on rice heat tolerance. Here, we observed the seedling characteristics of two contrasting cultivars (T11: heat-tolerant and T15: heat-sensitive) after different durations of 42 ffiC heat stress. The dynamic transcriptomic changes of the two cultivars were monitored after 0 min, 10 min, 30 min, 1 h, 4 h, and 10 h of stress. The results indicate that several pathways were rapidly responding to heat stress, such as protein processing in the endoplasmic reticulum, glycerophospholipid metabolism, and plant hormone signal transduction. Functional annotation and cluster analysis of differentially expressed genes at different stress times indicate that the tolerant cultivar responded more rapidly and intensively to heat stress compared to the sensitive cultivar. The MAPK signaling pathway was found to be the specific early-response pathway of the tolerant cultivar. Moreover, by combining data from a GWAS and RNA-seq analysis, we identified 27 candidate genes. The reliability of the transcriptome data was verified using RT-qPCR on 10 candidate genes and 20 genes with different expression patterns. This study provides valuable information for short-term thermotolerance response mechanisms active at the rice seedling stage and lays a foundation for breeding thermotolerant varieties via molecular breeding.

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