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Proteomic analysis of early salt stress responsive proteins in alfalfa roots and shoots

文献类型： 外文期刊

作者： Xiong, Junbo 1 ; Sun, Yan 2 ; Yang, Qingchuan 3 ; Tian, Hong 1 ; Zhang, Heshan 1 ; Liu, Yang 1 ; Chen, Mingxin 1 ;

作者机构： 1.Hubei Acad Agr Sci, Inst Anim & Vet Sci, Hubei Key Lab Anim Embryo & Mol Breeding, Yaoyuan 1, Wuhan 430017, Hubei, Peoples R China

2.China Agr Univ, Inst Grassland Sci, 2 West Rd, Beijing 100193, Peoples R China

3.Chinese Acad Agr Sci, Inst Anim Sci, West Rd 2, Beijing 100193, Peoples R China

关键词： NaCl stress;Medicago sativa root and shoot;Two-dimensional electrophoresis;Differentially abundant proteins

期刊名称：PROTEOME SCIENCE （ 影响因子：2.48； 五年影响因子：2.619 ）

ISSN： 1477-5956

年卷期： 2017 年 15 卷

页码：

收录情况： SCI

摘要： Background: Alfalfa (Medicago sativa) is the most extensively cultivated forage legume in the world, and salinity stress is the most problematic environmental factors limiting alfalfa production. To evaluate alfalfa tissue variations in response to salt stress, comparative physiological and proteomic analyses were made of salt responses in the roots and shoots of the alfalfa. Method: A two-dimensional gel electrophoresis (2-DE)-based proteomic technique was employed to identify the differentially abundant proteins (DAPs) from salt-treated alfalfa roots and shoots of the salt tolerance cultivars Zhongmu No 1 cultivar, which was subjected to a range of salt stress concentrations for 9 days. In parallel, REL, MAD and H2O2 contents, and the activities of antioxidant enzymes of shoots and roots were determinand. Result: Twenty-seven spots in the shoots and 36 spots in the roots that exhibited showed significant abundance variations were identified by MALDI-TOF-TOF MS. These DAPs are mainly involved in the biological processes of photosynthesis, stress and defense, carbohydrate and energy metabolism, second metabolism, protein metabolism, transcriptional regulation, cell wall and cytoskeleton metabolism, ion transpor, signal transduction. In parallel, physiological data were correlated well with our proteomic results. It is worth emphasizing that some novel salt-responsive proteins were identified, such as CP12, pathogenesis-related protein 2, harvest-induced protein, isoliquiritigenin 2'-O-methyltransferase. qRT-PCR was used to study the gene expression levels of the four above-mentioned proteins; four patterns are consistent with those of induced protein. Conclusion: The primary mechanisms underlying the ability of alfalfa seedlings to tolerate salt stress were photosynthesis, detoxifying and antioxidant, secondary metabolism, and ion transport. And it also suggests that the different tissues responded to salt-stress in different ways.

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