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Whole-genome bisulfite sequencing of X and Y sperm in Holstein bulls reveals differences in autosomal methylation status

文献类型： 外文期刊

作者： Shangguan, Aishao 1 ; Ding, Fengling 2 ; Ding, Rui 3 ; Sun, Wei 3 ; Li, Xihe 3 ; Bao, Xiangnan 3 ; Zhang, Tiezhu 4 ; Chi, Huihui 4 ; Xiong, Qi 1 ; Chen, Mingxin 1 ; Zhou, Yang 2 ; Zhang, Shujun 2 ;

作者机构： 1.Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Hubei Key Lab Anim Embryo Engn & Mol Breeding, Wuhan 430070, Peoples R China

2.Huazhong Agr Univ, Frontiers Sci Ctr Anim Breeding & Sustainable Prod, Minist Educ, Wuhan 430070, Peoples R China

3.Natl Ctr Technol Innovat Dairy, Hohhot 010020, Peoples R China

4.Inner Mongolia SaiKeXing Inst Breeding & Reprod Bi, Hohhot 010020, Peoples R China

关键词： Holstein bull; Whole-genome bisulfite sequencing; DNA methylation; X sperm and Y sperm; Epigenetics

期刊名称：BMC GENOMICS （ 影响因子：3.7； 五年影响因子：4.2 ）

ISSN： 1471-2164

年卷期： 2025 年 26 卷 1 期

页码：

收录情况： SCI

摘要： A comprehensive understanding of the molecular differences between X and Y sperm in Holstein bull semen is crucial for advancing sex control technologies. While previous studies have primarily focused on proteomic and transcriptomic differences, the genome-wide DNA methylation differences between these sperm types remains largely unexplored. In this study, we employed whole-genome bisulfite sequencing to systematically compare the autosomal methylation profiles of X and Y sperm. Although global methylation patterns showed remarkable consistency between the two sperm types, our localized comparative analysis revealed 12,175 differentially methylated regions mapping to 2,041 genes (differentially methylated genes, DMGs). Functional enrichment analysis of these DMGs revealed their involvement in essential biological processes, particularly in energy metabolism and membrane voltage regulation. Notably, SPA17 and CHCHD3, identified as hypermethylated genes in X sperm in this study, have also been reported to show lower protein expression levels in X sperm compared to Y sperm. Furthermore, we identified 28 DMGs functionally associated with spermatogenesis and 5 DMGs related to fertilization. Our findings lay the foundation for thorough understanding of molecular differences between X and Y sperm in bull, providing essential insights for the development of more advanced sex control technologies in the future.

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