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β-glucosidase, driven by porcine transthyretin promoter, specific expression in the liver of transgenic mice

文献类型： 外文期刊

作者： Zhang, Feng 2 ; Li, Yujiao 1 ; Xiong, Qi 2 ; Chai, Jin 1 ; Jiang, Siwen 1 ;

作者机构： 1.Huazhong Agr Univ, Key Lab Swine Genet & Breeding, Key Lab Agr Anim Genet Breeding & Reprod, Agr Minist,Minist Educ,Coll Anim Sci & Techn, Wuhan, Peoples R China

2.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Hubei Key Lab Anim Embryo Engn & Mol Breeding, Wuhan, Peoples R China

3.Shandong Prov Anim Prod Qual & Safety Ctr, Shandong Prov Livestock & Poultry Slaughtering Te, Jinan, Peoples R China

关键词： live; transgenic mice; TTR; beta-glucosidase

期刊名称：ANIMAL SCIENCE JOURNAL （ 影响因子：2.0； 五年影响因子：2.1 ）

ISSN： 1344-3941

年卷期： 2023 年 94 卷 1 期

页码：

收录情况： SCI

摘要： Under the background of food security, using non-grain feed instead of corn-soybean-based feed is an effective measure to alleviate the food-feed competition. While, non-grain feeds are often rich in fiber, which cannot be digested by non-ruminants. Producing heterologous enzymes in non-ruminants to improve cellulose utilization rate is a new research strategy by transgenic technology. In this study, porcine transthyretin (TTR) promoter, signal peptide-coding sequence (CDS), Saccharomycopsis fibuligera beta-glucosidase gene (BGL1)-CDS, 6xHis sequences fragments were fused into pGL3-control vector to generate transgenic vector. Then, transgenic mice were generated by pronuclear microinjection of the linearized expression vectors. Transgenic mice and their offspring were examined by PCR-based genotyping and copy number variation. Results showed that BGL1 was successfully integrated into the mouse genome and transmitted stably. Furthermore, reverse transcription-polymerase chain reaction (RT-PCR), Western blotting, and beta-glucosidase activity assay demonstrated that BGL1 was specifically expressed in the liver, and beta-glucosidase activity significantly increased. In addition, liver weight index, cellular morphology, and collagen fiber content of the liver showed that exogenous gene insertion did not cause any lesions to live. Taken together, our findings suggest that beta-glucosidase driven by TTR promoter was specifically expressed in the liver of transgenic mice.

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