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ACOX1, regulated by C/EBP alpha and miR-25-3p, promotes bovine preadipocyte adipogenesis

文献类型： 外文期刊

作者： Zhang, Feng 1 ; Xiong, Qi 1 ; Tao, Hu 1 ; Liu, Yang 1 ; Zhang, Nian 1 ; Li, Xiao-Feng 1 ; Suo, Xiao-Jun 1 ; Yang, Qian-Pi 1 ;

作者机构： 1.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Hubei Key Lab Anim Embryo Engn & Mol Breeding, Wuhan, Peoples R China

关键词： bovine; ACOX1; CEBP alpha; miR-25-3p; adipogenesis

期刊名称：JOURNAL OF MOLECULAR ENDOCRINOLOGY （ 影响因子：3.562； 五年影响因子：3.672 ）

ISSN： 0952-5041

年卷期： 2021 年 66 卷 3 期

页码：

收录情况： SCI

摘要： Acyl-coenzyme A oxidase 1 (ACOX1) is the first and rate-limiting enzyme in peroxisomal fatty acid beta-oxidation of fatty acids. Previous studies have reported that ACOX1 was correlated with the meat quality of livestock, while the role of ACOX1 in intramuscular adipogenesis of beef cattle and its transcriptional and post-transcriptional regulatory mechanisms remain unclear. In the present study, gain-of-function and loss-of-function assays demonstrated that ACOX1 positively regulated the adipogenesis of bovine intramuscular preadipocytes. The C/EBP alpha-binding sites in the bovine ACOX1 promoter region at -1142 to -1129 bp, -831 to -826 bp, and -303 to -298 bp were identified by promoter deletion analysis and site-directed mutagenesis. Electrophoretic mobility shift assays (EMSA) and chromatin immunoprecipitation (ChIP) further showed that these three regions are C/EBP alpha-binding sites, both in vitro and in vivo, indicating that C/EBP alpha directly interacts with the bovine ACOX1 promoter and inhibits its transcription. Furthermore, the results from bioinformatics analysis, dual luciferase assay, site-directed mutagenesis, qRT-PCR, and Western blotting demonstrated that miR-25-3p directly targeted the ACOX1 3'UTR (3'UTR). Taken together, our findings suggest that ACOX1, regulated by transcription factor C/EBP alpha and miR-25-3p, promotes adipogenesis of bovine intramuscular preadipocytes via regulating peroxisomal fatty acid beta-oxidation.

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