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Alternative splicing and tissue expression of CIB4 gene in sheep testis

文献类型： 外文期刊

作者： Yu, Yan 1 ; Zhang, Yuan 3 ; Song, Xuemei 4 ; Jin, Mei 5 ; Guan, Qun 2 ; Zhang, Qiyao 2 ; Li, Shangang; Wei, Caihong 1 ; L 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China

2.Shandong Univ Tradit Chinese Med, Reprod Med Ctr, Hosp 2, Jinan 250001, Peoples R China

3.China Agr Univ, Coll Anim Sci & Technol, Beijing 100193, Peoples R China

4.Zhejiang Acad Agr Sci, Inst Anim Sci, Hangzhou 310021, Zhejiang, Peoples R China

5.Liaoning Normal Univ, Coll Life Sci,

关键词： Sheep;CIB4;Gene cloning;Alternative splicing;Testis-specific expression gene

期刊名称：ANIMAL REPRODUCTION SCIENCE （ 影响因子：2.145； 五年影响因子：2.281 ）

ISSN： 0378-4320

年卷期： 2010 年 120 卷 1-4 期

页码：

收录情况： SCI

摘要： In this study, the sheep CIB4 cDNA was cloned from the small tail Han sheep by RT-PCR and RACE (rapid amplification of cDNA ends), and CIB4 cDNA and amino acid sequence were analyzed. Our results showed that the sheep CIB4 gene expressed two alternatively spliced variants L-CIB4 (long CIB4) and S-CIB4 (short CIB4). Sequence analysis indicated that the sheep CIB4 cDNA cloned (L-CIB4) was 745-bp in length (GenBank accession number: FJ039532) with 185 amino acids residues. The sheep CIB4 cDNA showed more than 72% of sequence identity, at the nucleotide level, to its equivalents in cattle, horse, chimpanzee, humans, mice and rats, while at the deduced protein level, the value increased to 79.6%. Semi-quantitative RT-PCR using total RNA from different tissues showed that CIB4 has a strong tissue-specific expression pattern in sheep. L-CIB4 expression level was shown to be no different in small tail Han sheep and the Dorset ram, but both were significantly different from the Texel (P < 0.05). Surprisingly, the short spliced form. S-CIB4, could only be detected in small tail Han sheep, suggesting that CIB4 may be linked in some way to the high fecundity of this breed. (C) 2010 Published by Elsevier B.V.

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