文献类型： 外文期刊
作者： Guo, Lijin 1 ; Huang, Weiling 1 ; Zhang, Siyu 1 ; Huang, Yulin 1 ; Xu, Yibin 1 ; Wu, Ruiquan 1 ; Fang, Xiang 1 ; Xu, Haiping 1 ; Nie, Qinghua 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Dept Anim Genet Breeding & Reprod, Lingnan Guangdong Lab Modern Agr, Guangzhou 510642, Peoples R China
2.South China Agr Univ, Coll Anim Sci, Dept Anim Genet Breeding & Reprod, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou 510642, Peoples R China
3.Minist Agr, Guangdong Prov Key Lab Agroanimal Genom & Mol Bre, Guangzhou 510642, Peoples R China
4.Minist Agr, Key Lab Chicken Genet Breeding & Reprod, Guangzhou 510642, Peoples R China
关键词： PROS1; chicken; adipogenesis; abdominal fat deposition; association
期刊名称：ANIMALS （ 影响因子：3.231； 五年影响因子：3.312 ）
ISSN： 2076-2615
年卷期： 2022 年 12 卷 16 期
页码：
收录情况： SCI
摘要： Simple Summary Low-fat meat is increasingly desired by the public due to the growing popularity of healthy diets, and the excessive accumulation of abdominal fat increases costs in the broiler breeding industry, all of which have encouraged breeding changes in the broiler industry. Investigating fat accumulation at a cellular level from a genetic perspective will help us understand gene-mediated abdominal fat accumulation in chickens. This study aimed to explore the role of the PROS1 gene in adipose cells and its application prospect in broiler breeding. Based on our findings, we found that the PROS1 gene can contribute to adipose cell proliferation and can reduce fat deposits at the cellular level, and its mutations are highly correlated with chicken fat traits. (1) Background: Excessive abdominal fat deposition in broilers not only causes feed waste but also leads to a series of metabolic diseases. It has gradually become a new breeding goal of the broiler industry to improve growth rates and to reduce abdominal fat rates. In a previous study, PROS1 was highly expressed in low-abdominal fat broilers, suggesting a potential role in broilers adipogenesis. However, the function of PROS1 in preadipocytes and its association with abdominal fat traits need to be characterized. (2) Methods: qRT-PCR and Western Blot were used to quantify gene expression at the RNA and protein levels; flow cytometry and EdU were carried out to detect cell proliferation; and a GLM analysis was used to determine the association between PROS1 SNPs and carcass traits. (3) Results: PROS1 was downregulated in high-abdominal fat chicken; PROS1 contributed preadipocyte proliferation but suppressed preadipocyte differentiation; and the SNPs in the PROS1 5 ' flank were significantly associated with the abdominal fat weight rate. (4) Conclusions: Chicken PROS1 is able to suppress adipogenesis, and its polymorphisms are associated with the abdominal fat weight rate, which can be considered the molecular markers for chicken breeding, indicating that PROS1 is an effective potential gene in regulating abdominal fat deposition.
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