您好，欢迎访问河南省农业科学院 机构知识库！
河南省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

RPA-CRISPR-Cas-Mediated Dual Lateral Flow Assay for the Point-of-Care Testing of HPV16 and HPV18

文献类型： 外文期刊

作者： Zhang, Kaini 1 ; Li, Qingmei 2 ; Wang, Kun 3 ; Zhang, Qiaoli 1 ; Ma, Chengkun 1 ; Yang, Guiwen 1 ; Xie, Yanxia 4 ; Mauk, Michael G. 5 ; Fu, Shanji 6 ; Chen, Lei 1 ;

作者机构： 1.Shandong Normal Univ, Coll Life Sci, Shandong Prov Key Lab Anim Resistance Biol, Jinan 250014, Shandong, Peoples R China

2.Henan Acad Agr Sci, Inst Anim Hlth Prevent & Control, Zhengzhou 450003, Henan, Peoples R China

3.Shandong First Med Univ, Shandong Prov Hosp, Dept Laws & Regulat, Jinan 250021, Peoples R China

4.Hongkui Biotechnol China Co Ltd, Jinan 271100, Shandong, Peoples R China

5.Univ Penn, Dept Mech Engn & Appl Mech, Philadelphia, PA 19104 USA

6.Shandong Univ, Qilu Hosp, Cheeloo Coll Med, Dept Clin Lab, Jinan 250012, Shandong, Peoples R China

期刊名称：BIOCONJUGATE CHEMISTRY （ 影响因子：3.9； 五年影响因子：4.5 ）

ISSN： 1043-1802

年卷期： 2024 年

页码：

收录情况： SCI

摘要： The incidence of cervical cancer caused by human papillomavirus (HPV) infection has increased in recent years. More than half of all cervical cancer cases are due to HPV16 and HPV18 infection, so HPV16 and HPV18 testing is essential to prevent cervical cancer. HPV testing is mainly carried out in hospitals, but it is subject to time and specialized medical facilities. On the other hand, home self-testing using simple diagnostics would present an attractive alternative due to privacy and flexibility with regard to time and place, provided sufficient sensitivity and specificity can be achieved. In this work, a dual lateral flow assay based on RPA-CRISPR-Cas12a/13a (named RC-LFA) for HPV detection was described. Taking advantage of the cleavage specificity of Cas12a and Cas13a, a CRISPR-Cas12a/Cas13a system was designed to detect HPV16 and HPV18. The lateral flow strip with two test lines was designed to suit the CRISPR-Cas12a/Cas13 system. RC-LFA achieves rapid and simultaneous detection of HPV16 and HPV18 with high specificity and sensitivity (10 copies/mu L) in about 40 min from the extraction of nucleic acid to an instrument-free readout. RC-LFA is user-friendly and instrument-free, making it a promising method for HPV self-tests at home.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台