Regulatory Effect of PGE2-EP2/EP4 Receptor Pathway on Staphylococcus aureus-Induced Inflammatory Factors in Dairy Cow Neutrophils
文献类型： 外文期刊
作者： Zhao, Yi 1 ; Wang, Chao 1 ; Liu, Bo 1 ; Zhang, Shuangyi 1 ; Wang, Yongfei 1 ; Qian, Yinghong 4 ; Gong, Zhiguo 1 ; Zhao, Jiamin 1 ; Yang, Xiaolin 1 ; Bai, Yuting 1 ; Mao, Wei 1 ;
作者机构： 1.Inner Mongolia Agr Univ, Coll Vet Med, Lab Vet Clin Pharmacol, 29 Erdosdong Rd, Hohhot 010011, Peoples R China
2.Inner Mongolia Agr Univ, Key Lab Clin Diag & Treatment Tech Anim Dis, Minist Agr, 29 Erdosdong Rd, Hohhot 010011, Peoples R China
3.Inner Mongolia Med Univ, Lab Anim Ctr, Hohhot 010030, Peoples R China
4.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot 010010, Peoples R China
关键词： neutrophils of cows; Staphylococcus aureus Rosenbach; prostaglandin E-2; EP2/EP4 receptor pathway; anti-inflammatory therapy
期刊名称：BIOMOLECULES （ 影响因子：4.8； 五年影响因子：5.6 ）
ISSN：
年卷期： 2025 年 15 卷 8 期
页码：
收录情况： SCI
摘要： Naturally occurring prostaglandin E-2 (PGE(2)) influences cytokine production regulation in bovine neutrophils exposed to Staphylococcus aureus Rosenbach. Here, we employed bovine neutrophils as the primary experimental system, and administered specific inhibitors targeting various receptors, which were subsequently exposed to S. aureus. Cytokine expression levels in dairy cow neutrophils induced by S. aureus via the endogenous PGE(2)-EP2/4 receptor pathway were investigated, and its effects on P38, extracellular signal-regulated kinase (ERK), P65 activation, and phagocytic function in Staphylococcus aureus Rosenbach-induced dairy cow neutrophils, were examined. Blocking cyclooxygenase-2 (COX-2) and microsomal prostaglandin E synthase-1 (mPGES-1) enzymes substantially decreased PGE(2) production and release in S. aureus-exposed bovine neutrophils. Cytokine output showed significant reduction compared to that in SA113-infected controls. Phosphorylation of P38, ERK, and P65 signaling molecules was depressed in the infected group. Pharmacological interference with EP2/EP4 receptors similarly diminished cytokine secretion and phosphorylation patterns of P38, ERK, and P65, with preserved cellular phagocytic function. During S. aureus infection of bovine neutrophils, COX-2 and mPGES-1 participated in controlling PGE(2) biosynthesis, and internally produced PGE(2) molecules triggered NF-kappa B and MAPK inflammatory pathways via EP2/EP4 receptor activation, later adjusting the equilibrium between cytokine types that promote or suppress inflammation. This signaling mechanism coordinated inflammatory phases through receptor-mediated processes.
- 相关文献
作者其他论文 更多>>
-
Targeting the mPGES-PGE2-EP4 pathway with MF63, MK886, and Grapiprant as a potential therapeutic strategy for Escherichia coli-induced endometritis in dairy cows
作者：Yang, Xiaolin;Guo, Lili;Gong, Pengfei;Wu, Jingze;Zhang, Shuangyi;Guo, Wenrui;Cao, Jinshan;Feng, Shuang;Mao, Wei;Yang, Xiaolin;Guo, Lili;Gong, Pengfei;Wu, Jingze;Zhang, Shuangyi;Guo, Wenrui;Cao, Jinshan;Feng, Shuang;Mao, Wei;Gong, Pengfei;Qian, Yinghong
关键词：Escherichia coli; Endometritis; Prostaglandin E 2; MF63; MK886; Grapiprant
-
Egg yolk-derived low-density lipoprotein: A potential drug delivery system to eradicate intracellular bacteria
作者：Zhao, Yi;Liu, Bo;Zhang, Shuang-yi;Wang, Yong-fei;Hasi, Su-rong;Gong, Zhi-guo;Zhao, Jia-min;Yang, Xiao-lin;Bai, Yu-ting;Cao, Jin-shan;Mao, Wei;Zhao, Yi;Liu, Bo;Zhang, Shuang-yi;Gong, Zhi-guo;Zhao, Jia-min;Yang, Xiao-lin;Cao, Jin-shan;Mao, Wei;Wang, Yong-fei;Qian, Ying-hong
关键词：Hen egg low-density lipoprotein; Ceftiofur; Intracellular drug delivery system; Staphylococcus aureus
-
Investigating the role of the mPGES-PGE2-EP4 pathway in Escherichia coli-induced mastitis in dairy cows: insights for non-antibiotic therapeutic strategies
作者：Yang, Xiaolin;Li, Xueqiang;Guo, Lili;Gong, Pengfei;Zhang, Shuangyi;Liu, Bo;Guo, Wenrui;Bao, Haixia;Mao, Wei;Yang, Xiaolin;Li, Xueqiang;Guo, Lili;Gong, Pengfei;Zhang, Shuangyi;Liu, Bo;Guo, Wenrui;Bao, Haixia;Mao, Wei;Gong, Pengfei;Qian, Yinghong
关键词：
Escherichia coli ; mastitis; alternative treatments to antibiotics; mPGES-1 inhibitors; EP4 receptor inhibitor -
An electric-driven maize seeding system: improving the quality of accelerate seeding using Tracking Differential Filtering-Optimal Tracking Control (TDF-OTC) method
作者：Zhai, Chengkun;Lu, Caiyun;Li, Hongwen;He, Jin;Wang, Qingjie;Wang, Chao;Bi, Jinshuo;Zhai, Chengkun;Lu, Caiyun;Li, Hongwen;He, Jin;Wang, Qingjie;Wang, Chao;Bi, Jinshuo;Jin, Wenlong;Chen, Liyu;Chang, Fangle
关键词：Accelerate seeding; Electric-driven seeding system; Tracking Differential Filtering-Optimal; Tracking Control; Nonlinear tracking differentiator; Linear quadratic tracker
-
Consumption of steam explosion and fermentation-pretreated corn stover affects the growth performance of sheep by shifting the rumen microbiota community structure
作者：Wang, Yong;Wang, Chao;Wei, Wurilege;Li, Changqing;Wang, Yong;Wang, Chao;Li, Changqing;Yin, Xuejiao;Hao, Kexing;Li, Yueqin;Gao, Sihui;Ji, Zeyu;Wang, Weiheng;Xie, Yuchun;Yin, Xuejiao;Hao, Kexing;Li, Yueqin;Gao, Sihui;Ji, Zeyu;Wang, Weiheng;Xie, Yuchun
关键词：steam explosion; corn stover; sheep; rumen microbiota; growth performance
-
The effect of taurochenodeoxycholic acid on the glucocorticoid receptor-mediated PLC-IP3-calcium pathway in synoviocytes derived from a rat adjuvant arthritis model
作者：Guanqia, Sangjide;Li, Peifeng;Cao, Jinshan;Mao, Wei;Wu, Jindi;Guanqia, Sangjide;Li, Peifeng;Cao, Jinshan;Bai, Fan;Qian, Yinghong;Mao, Wei
关键词：taurochenodeoxycholic acid; rat adjuvant arthritis model; glucocorticoid receptor; phospholipase C-inositol trisphosphate-Ca2+ signalling pathway
-
Prostaglandin E2 accumulation is closely associated with S. aureus-infected bovine endometritis
作者：Liu, Kun;Shen, Yuan;Zhang, Nan;Liu, Kun;Shen, Yuan;Wu, Jindi;Mao, Wei;Cao, Jinshan;Pei, Le;Qian, Yinghong
关键词：Endometritis; S. aureus infection; Prostaglandin E2; Immunology
