The effect of taurochenodeoxycholic acid on the glucocorticoid receptor-mediated PLC-IP3-calcium pathway in synoviocytes derived from a rat adjuvant arthritis model
文献类型： 外文期刊
作者： Wu, Jindi 2 ; Bai, Fan 4 ; Guanqia, Sangjide 1 ; Li, Peifeng 1 ; Cao, Jinshan 1 ; Qian, Yinghong 4 ; Mao, Wei 1 ;
作者机构： 1.Inner Mongolia Agr Univ, Coll Vet Med, Lab Vet Pharmacol, Hohhot, Peoples R China
2.Engn Inner Mongolia Agr Univ, Coll Food Sience, Hohhot, Peoples R China
3.Inner Mongolia Agr Univ, Key Lab Clin Diag & Treatment Tech Anim Dis, Minist Agr, Hohhot, Peoples R China
4.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot, Peoples R China
5.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot 010031, Peoples R China
关键词： taurochenodeoxycholic acid; rat adjuvant arthritis model; glucocorticoid receptor; phospholipase C-inositol trisphosphate-Ca2+ signalling pathway
期刊名称：VETERINARSKI ARHIV （ 影响因子：0.5； 五年影响因子：0.6 ）
ISSN： 0372-5480
年卷期： 2024 年 94 卷 1 期
页码：
收录情况： SCI
摘要： Taurochenodeoxycholic acid is one of the main active components of the bile acid pool and has important anti-inflammatory and immunomodulatory properties. This study aimed to explore the therapeutic effects and mechanism of action of taurochenodeoxycholic acid in inflammatory arthritis using a rat adjuvant arthritis model. Fibroblast-like synoviocytes were derived from rats with adjuvant arthritis and cultured using the adherent tissue explant method. Western blotting, enzyme-linked immunosorbent assay, and fluorescent probe-based methods were used to detect the effects of taurochenodeoxycholic acid on glucocorticoid receptor expression, phospholipase C protein levels, and inositol trisphosphate and intracellular free Ca2+ concentrations in primary rat fibroblast-like synoviocytes. Taurochenodeoxycholic acid significantly induced the expression of glucocorticoid receptor (P<0.05) and phospholipase C (P<0.05) and enhanced phospholipase C phosphorylation. Moreover, taurochenodeoxycholic acid significantly increased the levels of inositol trisphosphate (P<0.05) and intracellular free Ca2+ concentrations. Our results suggest that taurochenodeoxycholic acid activates the phospholipase C-inositol trisphosphate-Ca2+ signalling pathway by increasing glucocorticoid receptor expression. These findings provide a theoretical foundation for future studies on the molecular mechanisms underlying taurochenodeoxycholic acid-based treatment of adjuvant arthritis. In conclusion, taurochenodeoxycholic acid exerted anti-inflammatory and immunomodulatory effects by enhancing glucocorticoid receptor expression via non-genomic signalling.
- 相关文献
作者其他论文 更多>>
-
Prostaglandin E2 accumulation is closely associated with S. aureus-infected bovine endometritis
作者：Liu, Kun;Shen, Yuan;Zhang, Nan;Liu, Kun;Shen, Yuan;Wu, Jindi;Mao, Wei;Cao, Jinshan;Pei, Le;Qian, Yinghong
关键词：Endometritis; S. aureus infection; Prostaglandin E2; Immunology
-
A Single-Cell Atlas of an Early Mongolian Sheep Embryo
作者：He, Tingyi;Guo, Wenrui;Su, Hong;Dou, Aolei;Chen, Lu;Ma, Teng;Liu, Moning;Su, Budeng;Qi, Wangmei;Li, Haijun;Mao, Wei;Wang, Xiumei;Cao, Guifang;He, Tingyi;Yang, Yanyan;Yang, Guang;Li, Xihe;Song, Yongli;Yang, Guang;Li, Xihe;Song, Yongli;Su, Jie
关键词：Mongolian sheep; embryo; scRNA-seq; Hippo signaling pathway
-
Staphylococcus aureus increases Prostaglandin E-2 secretion in cow neutrophils by activating TLR2, TLR4, and NLRP3 inflammasome signaling pathways
作者：Zhang, Kai;Jia, Yan;Jiang, Xueying;Zhang, Shuangyi;Liu, Bo;Cao, Jinshan;Mao, Wei;Zhang, Kai;Jia, Yan;Jiang, Xueying;Zhang, Shuangyi;Liu, Bo;Cao, Jinshan;Mao, Wei;Qian, Yinghong;Song, Yongli
关键词：Staphylococcus aureus; Prostaglandin E-2; neutrophils; toll-like receptor 2; toll-like receptor 4; NLR pyrin domain-containing 3
-
Anti-inflammatory effects of the prostaglandin D-2/prostaglandin DP1 receptor and lipocalin-type prostaglandin D-2 synthase/prostaglandin D-2 pathways in bacteria-induced bovine endometrial tissue
作者：Wu, Jindi;Borjigin, Gerelt;Wu, Jindi;Mao, Wei;Liu, Bo;Yang, Xiaolin;Zhang, Jing;Li, Tingting;Cao, Jinshan;Wu, Jindi;Mao, Wei;Liu, Bo;Yang, Xiaolin;Zhang, Jing;Li, Tingting;Cao, Jinshan;Bai, Fan
关键词：DP1 receptor; Escherichia coli; Staphylococcus aureus; anti-inflammatory effects; bovine endometrial tissue; L-PGDS; PGD(2) pathway
-
Live S. aureus and heat-killed S. aureus induce different inflammation-associated factors in bovine endometrial tissue in vitro
作者：Liu, Kun;Mao, Wei;Liu, Bo;Li, Tingting;Wu, Jindi;Fu, Changqi;Shen, Yuan;Pei, Le;Cao, Jinshan;Liu, Kun;Mao, Wei;Liu, Bo;Li, Tingting;Wu, Jindi;Fu, Changqi;Shen, Yuan;Cao, Jinshan;Pei, Le
关键词：S; aureus; bovine endometrial tissue; pro-inflammatory factors; PGE2
-
Prostaglandin E2 promotes Staphylococcus aureus infection via EP4 receptor in bovine endometrium
作者：Liu, Kun;Mao, Wei;Liu, Bo;Li, Tingting;Wang, Xinfei;Cao, Jinshan;Liu, Kun;Mao, Wei;Liu, Bo;Li, Tingting;Wang, Xinfei;Cao, Jinshan;Liu, Kun;Wang, Fenglong;Pei, Le
关键词：Bovine endometritis; Prostaglandin E2; S; aureus; Infection; EP4 receptor
-
Mycoplasma ovipneumoniae-derived lipid-associated membrane proteins induce cytokine secretion in mouse peritoneal macrophages through TLR2 signalling
作者：Bai, Fan;Wang, Xiaohui;Shi, Xiaona;Lv, Tianxing;Wang, Yanfang;Hao, Yongqing;Wu, Jindi;Liu, Bo;Wu, Jindi;Liu, Bo;Bai, Fan
关键词：Mycoplasma ovipneumoniae; TLR2; Lipid-associated membrane protein; Inflammasome; Cytokines
