您好，欢迎访问内蒙古农牧业科学院 机构知识库！
内蒙古农牧业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

The effect of taurochenodeoxycholic acid on the glucocorticoid receptor-mediated PLC-IP3-calcium pathway in synoviocytes derived from a rat adjuvant arthritis model

文献类型： 外文期刊

作者： Wu, Jindi 2 ; Bai, Fan 4 ; Guanqia, Sangjide 1 ; Li, Peifeng 1 ; Cao, Jinshan 1 ; Qian, Yinghong 4 ; Mao, Wei 1 ;

作者机构： 1.Inner Mongolia Agr Univ, Coll Vet Med, Lab Vet Pharmacol, Hohhot, Peoples R China

2.Engn Inner Mongolia Agr Univ, Coll Food Sience, Hohhot, Peoples R China

3.Inner Mongolia Agr Univ, Key Lab Clin Diag & Treatment Tech Anim Dis, Minist Agr, Hohhot, Peoples R China

4.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot, Peoples R China

5.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot 010031, Peoples R China

关键词： taurochenodeoxycholic acid; rat adjuvant arthritis model; glucocorticoid receptor; phospholipase C-inositol trisphosphate-Ca2+ signalling pathway

期刊名称：VETERINARSKI ARHIV （ 影响因子：0.5； 五年影响因子：0.6 ）

ISSN： 0372-5480

年卷期： 2024 年 94 卷 1 期

页码：

收录情况： SCI

摘要： Taurochenodeoxycholic acid is one of the main active components of the bile acid pool and has important anti-inflammatory and immunomodulatory properties. This study aimed to explore the therapeutic effects and mechanism of action of taurochenodeoxycholic acid in inflammatory arthritis using a rat adjuvant arthritis model. Fibroblast-like synoviocytes were derived from rats with adjuvant arthritis and cultured using the adherent tissue explant method. Western blotting, enzyme-linked immunosorbent assay, and fluorescent probe-based methods were used to detect the effects of taurochenodeoxycholic acid on glucocorticoid receptor expression, phospholipase C protein levels, and inositol trisphosphate and intracellular free Ca2+ concentrations in primary rat fibroblast-like synoviocytes. Taurochenodeoxycholic acid significantly induced the expression of glucocorticoid receptor (P<0.05) and phospholipase C (P<0.05) and enhanced phospholipase C phosphorylation. Moreover, taurochenodeoxycholic acid significantly increased the levels of inositol trisphosphate (P<0.05) and intracellular free Ca2+ concentrations. Our results suggest that taurochenodeoxycholic acid activates the phospholipase C-inositol trisphosphate-Ca2+ signalling pathway by increasing glucocorticoid receptor expression. These findings provide a theoretical foundation for future studies on the molecular mechanisms underlying taurochenodeoxycholic acid-based treatment of adjuvant arthritis. In conclusion, taurochenodeoxycholic acid exerted anti-inflammatory and immunomodulatory effects by enhancing glucocorticoid receptor expression via non-genomic signalling.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：内蒙古农牧业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台