Prostaglandin E2 promotes Staphylococcus aureus infection via EP4 receptor in bovine endometrium
文献类型： 外文期刊
作者： Liu, Kun 1 ; Mao, Wei 1 ; Liu, Bo 1 ; Li, Tingting 1 ; Wang, Xinfei 1 ; Pei, Le 4 ; Cao, Jinshan 1 ; Wang, Fenglong 3 ;
作者机构： 1.Inner Mongolia Agr Univ, Coll Vet Med, Lab Vet Pharmacol, 306 Zhaowuda Rd, Saihan Dist 010018, Hohhot, Peoples R China
2.Minist Agr, Key Lab Clin Diag & Treatment Tech Anima Dis, Hohhot, Peoples R China
3.Inner Mongolia Agr Univ, Coll Vet Med, Lab Vet Pathol, 306 Zhaowuda Rd, Saihan Dist 010018, Hohhot, Peoples R China
4.Inner Mongolia Acad Agr & Anim Husb Sci, Vet Res Inst, Hohhot, Peoples R China
关键词： Bovine endometritis; Prostaglandin E2; S; aureus; Infection; EP4 receptor
期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.664 ）
ISSN： 0882-4010
年卷期： 2021 年 158 卷
页码：
收录情况： SCI
摘要： Prostaglandin E2 (PGE2) enhances Staphylococcus aureus infection but its mechanism is not well understood. Here, we examined the effect of PGE2 on Staphylococcal Protein A (SPA) expression in bovine endometrium and determined the role of select PGE2 receptors (i.e., EP2 and EP4) in adhesion and internalization of S. aureus. S. aureus isolate SA113 was used for in vitro infection of bovine endometrial tissues and epithelial cells, with treatment conditions consisting of untreated control, SA113 treatment, SA113 + PGE2, SA113 + PGE2 + EP2 receptor antagonist (AH-6809), and SA113 + PGE2 + EP4 receptor antagonist (AH-23848). Immunofluorescence assay revealed that PGE2 could promote SPA expression in S. aureus-infected bovine endometrial tissues. PGE2 also enhanced the adhesion and internalization of S. aureus in bovine endometrial cells. The addition of EP4 antagonist, but not the EP2 antagonist, abrogated the ability of PGE2 to promote S. aureus SPA expression, adhesion, and internalization in endometrial cells. Our findings suggest that S. aureus infection in the endometrium is enhanced by PGE2 through the EP4 receptor. This result is essential for the development of new approach to treating S. aureus infection, such as the application of EP4 antagonist as an adjunct drug treatment.
- 相关文献
作者其他论文 更多>>
-
Targeting the mPGES-PGE2-EP4 pathway with MF63, MK886, and Grapiprant as a potential therapeutic strategy for Escherichia coli-induced endometritis in dairy cows
作者：Yang, Xiaolin;Guo, Lili;Gong, Pengfei;Wu, Jingze;Zhang, Shuangyi;Guo, Wenrui;Cao, Jinshan;Feng, Shuang;Mao, Wei;Yang, Xiaolin;Guo, Lili;Gong, Pengfei;Wu, Jingze;Zhang, Shuangyi;Guo, Wenrui;Cao, Jinshan;Feng, Shuang;Mao, Wei;Gong, Pengfei;Qian, Yinghong
关键词：Escherichia coli; Endometritis; Prostaglandin E 2; MF63; MK886; Grapiprant
-
Egg yolk-derived low-density lipoprotein: A potential drug delivery system to eradicate intracellular bacteria
作者：Zhao, Yi;Liu, Bo;Zhang, Shuang-yi;Wang, Yong-fei;Hasi, Su-rong;Gong, Zhi-guo;Zhao, Jia-min;Yang, Xiao-lin;Bai, Yu-ting;Cao, Jin-shan;Mao, Wei;Zhao, Yi;Liu, Bo;Zhang, Shuang-yi;Gong, Zhi-guo;Zhao, Jia-min;Yang, Xiao-lin;Cao, Jin-shan;Mao, Wei;Wang, Yong-fei;Qian, Ying-hong
关键词：Hen egg low-density lipoprotein; Ceftiofur; Intracellular drug delivery system; Staphylococcus aureus
-
Investigating the role of the mPGES-PGE2-EP4 pathway in Escherichia coli-induced mastitis in dairy cows: insights for non-antibiotic therapeutic strategies
作者：Yang, Xiaolin;Li, Xueqiang;Guo, Lili;Gong, Pengfei;Zhang, Shuangyi;Liu, Bo;Guo, Wenrui;Bao, Haixia;Mao, Wei;Yang, Xiaolin;Li, Xueqiang;Guo, Lili;Gong, Pengfei;Zhang, Shuangyi;Liu, Bo;Guo, Wenrui;Bao, Haixia;Mao, Wei;Gong, Pengfei;Qian, Yinghong
关键词：
Escherichia coli ; mastitis; alternative treatments to antibiotics; mPGES-1 inhibitors; EP4 receptor inhibitor -
Inoculation with arbuscular mycorrhizal fungi at the seedling stage of potatoes improves phosphorus use efficiency
作者：Liu, Xiaoyu;Yu, Jing;Qin, Yonglin;Shi, Xiaohua;Liu, Kun;Jia, Liguo;Fan, Mingshou;Liu, Xiaoyu;Wei, Jie
关键词：AMF infection rate; potato growth; soil mycorrhizal hyphal density; phosphorus uptake; tuber yield
-
Regulatory Effect of PGE2-EP2/EP4 Receptor Pathway on Staphylococcus aureus-Induced Inflammatory Factors in Dairy Cow Neutrophils
作者：Zhao, Yi;Wang, Chao;Liu, Bo;Zhang, Shuangyi;Wang, Yongfei;Gong, Zhiguo;Zhao, Jiamin;Yang, Xiaolin;Bai, Yuting;Mao, Wei;Zhao, Yi;Wang, Chao;Liu, Bo;Zhang, Shuangyi;Gong, Zhiguo;Zhao, Jiamin;Yang, Xiaolin;Wang, Yongfei;Qian, Yinghong
关键词：neutrophils of cows; Staphylococcus aureus Rosenbach; prostaglandin E-2; EP2/EP4 receptor pathway; anti-inflammatory therapy
-
The effect of taurochenodeoxycholic acid on the glucocorticoid receptor-mediated PLC-IP3-calcium pathway in synoviocytes derived from a rat adjuvant arthritis model
作者：Guanqia, Sangjide;Li, Peifeng;Cao, Jinshan;Mao, Wei;Wu, Jindi;Guanqia, Sangjide;Li, Peifeng;Cao, Jinshan;Bai, Fan;Qian, Yinghong;Mao, Wei
关键词：taurochenodeoxycholic acid; rat adjuvant arthritis model; glucocorticoid receptor; phospholipase C-inositol trisphosphate-Ca2+ signalling pathway
-
Prostaglandin E2 accumulation is closely associated with S. aureus-infected bovine endometritis
作者：Liu, Kun;Shen, Yuan;Zhang, Nan;Liu, Kun;Shen, Yuan;Wu, Jindi;Mao, Wei;Cao, Jinshan;Pei, Le;Qian, Yinghong
关键词：Endometritis; S. aureus infection; Prostaglandin E2; Immunology
