文献类型： 外文期刊

作者： Xu, Pengfei ¹ ; Zhang, Wenting ⁴ ; Wang, Xuan ⁵ ; Zhu, Yantao ⁶ ; Liang, Wanqi ¹ ; He, Yuke ² ; Yu, Xiang ¹ ;

作者机构： 1.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Joint Int Res Lab Metab & Dev Sci, Shanghai, Peoples R China

2.Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci, Natl Key Lab Plant Mol Genet, Shanghai, Peoples R China

3.Univ Chinese Acad Sci, Beijing, Peoples R China

4.Guangdong Acad Agr Sci, Crops Res Inst, Guangdong Prov Key Lab Crops Genet & Improvement, Guangzhou, Peoples R China

5.Shanghai Normal Univ, Coll Life Sci, Shanghai Key Lab Plant Mol Sci, Shanghai, Peoples R China

6.Hybrid Rape Res Ctr Shaanxi Prov, Yangling, Shaanxi, Peoples R China

7.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Joint Int Res Lab Metab & Dev Sci, Shanghai 200240, Peoples R China

8.Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China

关键词： Brassica; GWAS; low temperature; miR1885; small RNA sequencing

期刊名称：PLANT CELL AND ENVIRONMENT （ 影响因子：7.3； 五年影响因子：8.1 ）

ISSN： 0140-7791

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Brassica crops include various edible vegetable and plant oil crops, and their production is limited by low temperature beyond their tolerant capability. The key regulators of low-temperature resistance in Brassica remain largely unexplored. To identify posttranscriptional regulators of plant response to low temperature, we performed small RNA profiling, and found that 16 known miRNAs responded to cold treatment in Brassica rapa. The cold response of seven of those miRNAs were further confirmed by qRT-PCR and/or northern blot analyses. In parallel, a genome-wide association study of 220 accessions of Brassica napus identified four candidate MIRNA genes, all of which were cold-responsive, at the loci associated with low-temperature resistance. Specifically, these large-scale data analyses revealed a link between miR1885 and the plant response to low temperature in both B. rapa and B. napus. Using 5 & PRIME; rapid amplification of cDNA ends approach, we validated that miR1885 can cleave its putative target gene transcripts, Bn.TIR.A09 and Bn.TNL.A03, in B. napus. Furthermore, overexpression of miR1885 in Semiwinter type B. napus decreased the mRNA abundance of Bn.TIR.A09 and Bn.TNL.A03 and resulted in increased sensitivity to low temperature. Knocking down of miR1885 in Spring type B. napus led to increased mRNA abundance of its targets and improved rapeseed tolerance to low temperature. Together, our results suggested that the loci of miR1885 and its targets could be potential candidates for the molecular breeding of low temperature-tolerant Spring type Brassica crops.