您好,欢迎访问中国水产科学研究院 机构知识库!

Soybean saponin modulates nutrient sensing pathways and metabolism in zebrafish

文献类型: 外文期刊

作者: Tian, Juan 1 ; Wang, Kaidi 1 ; Wang, Xuan 1 ; Wen, Hua 2 ; Zhou, Huihui 1 ; Liu, Chengdong 1 ; Mai, Kangsen 1 ; He, Gen 1 ;

作者机构: 1.Ocean Univ China, Key Lab Aquaculture Nutr, Minist Agr, Qingdao 266003, Peoples R China

2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat, Minist Agr, Wuhan 430223, Hubei, Peoples R China

3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peo

关键词: Soybean saponin;Zebrafish;TOR pathway;IGF;Metabolic enzymes

期刊名称:GENERAL AND COMPARATIVE ENDOCRINOLOGY ( 影响因子:2.822; 五年影响因子:2.772 )

ISSN: 0016-6480

年卷期: 2018 年 257 卷

页码:

收录情况: SCI

摘要: Soybean saponin (SA) is known as a major anti-nutritional factor that causes metabolic disturbances and growth reduction in fish. However, the mechanisms underlying these effects were far from fully understood. In particular, the influences of SA on nutrient sensing and downstream metabolic pathways remain largely unexplored. Using zebrafish as an animal model, this study was conducted to examine the phenotypic and molecular responses after dietary SA treatment for 2 weeks. SA at both 5 and 10 g/kg diet levels significantly reduced growth performance and feed efficiency, and damaged the morphology of the intestinal mucosa. SA stimulated AMP-activated protein kinase but reduced target of rapamycin (TOR) activities in both feeding trial and cellular studies. Furthermore, SA increased the mRNA expressions of growth axis genes including growth hormone, insulin-like growth factor 1, growth hormone receptor A, and growth hormone receptor B, but decreased insulin-like growth factor-binding protein 2 at both mRNA and protein levels. SA also increased the expressions of key metabolic enzymes involved in glutamine synthetase, glutamate dehydrogenase and lipolysis, hormone-sensitive lipase and lipoprotein lipase. Our results demonstrated that SA modulated nutrient sensing pathways and metabolism, thus provide new aspects on the explanation of the physiological effects of SA. (C) 2017 Elsevier Inc. All rights reserved.

  • 相关文献

[1]Effects of postprandial starvation on mRNA expression of endocrine-, amino acid and peptide transporter-, and metabolic enzyme-related genes in zebrafish (Danio rerio). Tian, Juan,He, Gen,Mai, Kangsen,Liu, Chengdong,Tian, Juan.

[2]CS5931, a Novel Polypeptide in Ciona savignyi, Represses Angiogenesis via Inhibiting Vascular Endothelial Growth Factor (VEGF) and Matrix Metalloproteinases (MMPs). Liu, Ge,Wu, Ning,Liu, Ge,Liu, Ming,Wei, Jianteng,Huang, Haijuan,Zhang, Yuyan,Zhao, Jin,Xiao, Lin,Zheng, Lanhong,Lin, Xiukun. 2014

[3]Proteomic profile and toxicity pathway analysis in zebrafish embryos exposed to bisphenol A and di-n-butyl phthalate at environmentally relevant levels. Dong, Xing,Qiu, Xuchun,Xu, Hai,Wu, Xiangyang,Qiu, Xuchun,Meng, Shunlong,Yang, Ming. 2018

[4]Identification of a virulence-related surface protein XF in piscine Streptococcus agalactiae by pre-absorbed immunoproteomics. Liu, Guangjin,Zhang, Wei,Liu, Yongjie,Yao, Huochun,Lu, Chengping,Xu, Pao. 2014

[5]Cloning, characterization and expression analysis of interleukin-10 from the zebrarish (Danio rerion). Zhang, DC,Shao, YQ,Huang, YQ,Jiang, SG. 2005

[6]Generation of Tg(cyp1a:gfp) Transgenic Zebrafish for Development of a Convenient and Sensitive In Vivo Assay for Aryl Hydrocarbon Receptor Activity. Xu, Hongyan,Li, Caixia,Li, Yan,Ng, Grace Hwee Boon,Liu, Chunsheng,Zhang, Xiaoyan,Gong, Zhiyuan,Xu, Hongyan,Liu, Chunsheng.

[7]The enantioselective toxicity and oxidative stress of beta-cypermethrin on zebrafish. Mu, Xiyan,Shen, Gongming,Huang, Ying,Luo, Jianbo,Li, Yingren,Zhu, Lizhen,Wang, Chengju,Li, Xuefeng,Qi, Suzhen.

[8]Dexamethasone-induced hepatomegaly and steatosis in larval zebrafish. Yin, Guojun,Kitazawa, Takio,Teraoka, Hiroki,Yin, Guojun,Cao, Liping,Du, Jinliang,Jia, Rui,Kubota, Akira.

[9]Assessment of Sediment Quality of Yangtze River Estuary Using Zebrafish (Danio rerio) Embryos. Wu, Lingling,Chen, Ling,Zhang, Yalei,Zhao, Jianfu,Gao, Hongwen,Wu, Lingling,Hou, Junli.

[10]Bioinformatic Analyses of CCCH-Zinc Finger Family in Zebrafish (Danio rerio). Shui, Yan,Xu, Zeng-Hong,Zhou, Xin,Zhou, Xin. 2014

[11]Aroclor 1254 and BDE-47 inhibit dopaminergic function manifesting as changes in locomotion behaviors in zebrafish embryos. Tanaka, Yasuaki,Fujiwara, Mari,Shindo, Asako,Kitazawa, Takio,Teraoka, Hiroki,Shindo, Asako,Yin, Guojun. 2018

[12]Characterization and toxicology evaluation of chitosan nanoparticles on the embryonic development of zebrafish, Danio rerio. Wang, Yanbo,Zhou, Jinru,Liu, Lin,Fu, Linglin,Huang, Changjiang,Zhou, Deqing.

[13]Derivation of a pluripotent embryonic cell line from red sea bream blastulas. Chen, SL,Ye, HQ,Sha, ZX,Hong, Y.

[14]The developmental effect of difenoconazole on zebrafish embryos: A mechanism research. Mu, Xiyan,Chai, Tingting,Zhu, Lizhen,Li, Xuefeng,Wang, Chengju,Mu, Xiyan,Huang, Ying,Shen, Gongming,Li, Yingren,Wang, Kai.

[15]Molecular cloning and characterization of the Myf5 gene in sea perch (Lateolabrax japonicus). Ye, Han-Qing,Chen, Song-Lin,Xu, Jian-Yong.

[16]Development of a positive-negative selection procedure for gene targeting in fish cells. Chen, SL,Hong, YH,Schartl, M.

作者其他论文 更多>>