Isolation, Identification, and Detection, of the Virulence Factor of Vibrio cholerae in Grass Carp
文献类型: 外文期刊
作者: Teng, Tao 1 ; Liang, Liguo 2 ; Xie, Jun 2 ;
作者机构: 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi, Peoples R China
2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Minist Agr, Key Lab Freshwater Fisheries & Germplasm Resource, Wuxi, Peoples R China
关键词: Grass carp;Vibrio cholera;isolation;identification;detection of virulence factor
期刊名称:ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH ( 影响因子:0.287; 五年影响因子:0.593 )
ISSN: 0792-156X
年卷期: 2015 年 67 卷
页码:
收录情况: SCI
摘要: In this study, we examined the mass mortality of grass carp (Ctenopharyngodon idellus) caused by Vibrio cholerae in Jiangsu Province. We evaluated the phenotypic, morphological, physiological, biochemical, and virulence factor characteristics as well as the drug sensitivity of the CY-1 strain isolated from the liver, spleen, and kidney, of dying grass carp. The 16S rRNA gene (GenBank accession number: KF386612) was amplified and compared with sequences deposited in GenBank. A phylogenetic tree analysis showed that the isolated pathogen was V. cholerae and that it was most closely related to V. cholerae (GenBank accession number: JN555611). Artificially infected grass carp that died exhibited the same disease symptoms as those of naturally infected fish. An amplified band in the corresponding 564-bp position was detected as the virulence factor, and it was the same as that of the V. cholerae virulence factor ctxA. Drug sensitivity tests showed that V. cholerae was resistant to penicillin, tobramycin, cotrimoxazole, and amoxicillin, and was highly sensitive to cefuroxime, amikacin, tetracycline, and nitrofurantoin. These could possibly be used in the treatment of this disease. The findings of this study can be used as a reference for disease control and health management in the cultivation of C. Idellus.
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