您好,欢迎访问中国水产科学研究院 机构知识库!

p38 MAPK Signaling Mediates Mitochondrial Apoptosis in Cancer Cells Induced by Oleanolic Acid

文献类型: 外文期刊

作者: Liu, Jia 1 ; Wu, Ning 1 ; Ma, Lei-Na 3 ; Zhong, Jia-Teng 6 ; Liu, Ge 1 ; Zheng, Lan-Hong 2 ; Lin, Xiu-Kun 1 ;

作者机构: 1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China

3.Ocean Univ China, Sch Med & Pharm, Dept Mol Biol, Qingdao, Peoples R China

4.Univ Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

5.Capital Med Univ, Dept Pharmacol, Beijing, Peoples R China

6.Jilin Univ, Norman Bethune Coll Med, Dept Pathophysiol, Changchun 130023, Peoples R China

关键词: Oleanolic acid;apoptosis;p38 MAPK;cancer;ROS

期刊名称:ASIAN PACIFIC JOURNAL OF CANCER PREVENTION ( 影响因子:2.514; 五年影响因子:2.267 )

ISSN: 1513-7368

年卷期: 2014 年 15 卷 11 期

页码:

收录情况: SCI

摘要: Oleanolic acid (OA) is a nutritional component widely distributed in various vegetables. Although it has been well recognized for decades that OA exerts certain anti-tumor activity by inducing mitochondria-dependent apoptosis, it is still unclear that what molecular signaling is responsible for this effect. In this study, we employed cancer cell lines, A549, BXPC-3, PANC-1 and U2OS to elucidate the molecular mechanisms underlying OA antitumor activity. We found that activation of MAPK pathways, including p-38 MAPK, JNK and ERK, was triggered by OA in both a dose and time-dependent fashion in all the tested cancer cells. Activation was accompanied by cleavage of caspases and PARP as well as cytochrome C release. SB203580 (p38 MAPK inhibitor), but not SP600125 (JNK inhibitor) and U0126 (ERK inhibitor), rescued the pro-apoptotic effect of OA on A549 and BXPC-3 cells. OA induced p38 MAPK activation promoted mitochondrial translocation of Bax and Bim, and inhibited Bcl-2 function by enhancing their phosphorylation. OA can induce reactive oxygen species (ROS)-dependent ASK1 activation, and this event was indispensable for p38 MAPK-dependent apoptosis in cancer cells. In vivo, p38 MAPK knockdown A549 tumors proved resistant to the growth-inhibitory effect of OA. Collectively, we elucidated that activation of ROS/ASK1/p38 MAPK pathways is responsible for the apoptosis stimulated by OA in cancer cells. Our finding can contribute to a better understanding of molecular mechanisms underlying the antitumor activity of nutritional components.

  • 相关文献

[1]Oleanolic acid induces protective autophagy in cancer cells through the JNK and mTOR pathways. Liu, Jia,Wu, Ning,Liu, Ge,Lin, Xiukun,Zheng, Lanhong,Liu, Jia,Liu, Ge,Lin, Xiukun,Zhong, Jiateng.

[2]ERK inhibition sensitizes cancer cells to oleanolic acid-induced apoptosis through ERK/Nrf2/ROS pathway. Liu, Jia,Ma, Leina,Chen, Xiao,Wang, Jianxun,Yu, Tao,Gong, Ying,Ma, Aiguo,Liang, Hui,Ma, Leina,Zheng, Lanhong. 2016

[3]Cytotoxic effects and apoptosis induction of enrofloxacin in hepatic cell line of grass carp (Ctenopharyngodon idellus). Liu, Bo,Ge, Xianping,Xie, Jun,Xu, Pao,Liu, Bo,Cui, Yanting,Ge, Xianping,Xie, Jun,Xu, Pao,Brown, Paul B..

[4]The effect of emodin on cytotoxicity, apoptosis and antioxidant capacity in the hepatic cells of grass carp (Ctenopharyngodon idellus). Cui, Yan-ting,Liu, Bo,Xie, Jun,Xu, Pao,Habte-Tsion, H. -Michael,Zhang, Yuan-yuan,Cui, Yan-ting,Liu, Bo,Xie, Jun,Xu, Pao,Habte-Tsion, H. -Michael,Zhang, Yuan-yuan.

[5]Aplysin Sensitizes Cancer Cells to TRAIL by Suppressing P38 MAPK/Survivin Pathway. Liu, Jia,Wu, Ning,Liu, Ge,Lin, Xiukun,Liu, Jia,Liu, Ge,Ma, Leina,Zheng, Lanhong,Lin, Xiukun. 2014

[6]Oleanolic acid inhibits proliferation and invasiveness of Kras-transformed cells via autophagy. Liu, Jia,Zheng, Lanhong,Liu, Jia,Wu, Ning,Liu, Ge,Lin, Xiukun,Ma, Leina,Wang, Bin,Zhao, Youguang,Lin, Xiukun.

[7]Oleanolic Acid Induces Metabolic Adaptation in Cancer Cells by Activating the AMP-Activated Protein Kinase Pathway. Liu, Jia,Wu, Ning,Liu, Ge,Lin, Xiukun,Zheng, Lanhong,Liu, Jia,Liu, Ge,Ma, Leina,Zhong, Jiateng,Lin, Xiukun.

[8]Effects of lactobacillus strains on colon cancer cell proliferation and cell cycle blockage. Shumei Wang,Lanwei Zhang,Wei Gu. 2012

[9]Effect of polychlorinated biphenyls on osmoregulatory response and apoptosis in GIFT tilapia, Oreochromis niloticus. Zheng, Y.,Qiu, L.,Fan, L.,Song, C.,Meng, S.,Chen, J.,Zheng, Y.,Qiu, L.,Fan, L.,Song, C.,Meng, S.,Chen, J.,Zheng, Y.,Chen, J.. 2016

[10]Toxic dinoflagellate Alexandrium tamarense induces oxidative stress and apoptosis in hepatopancreas of shrimp (Fenneropenaeus chinensis). Liang Zhongxiu,Ren Hai,Liang Zhongxiu,Li Jian,Li Jitao,Tan Zhijun,Zhao Fazhen. 2014

[11]Toxic threshold of dietary microcystin (-LR) for quart medaka. Deng, Dong-Fang,Teh, Foo-Ching,Teh, Swee J.,Deng, Dong-Fang,Teh, Foo-Ching,Teh, Swee J.,Zheng, Keke,Lehman, Peggy W.. 2010

[12]A polypeptide from Chlamys farreri inhibits UVB-induced HaCaT cells apoptosis via the Apaf-1/caspase-9 and Smac/XIAP signaling pathway. Liu Xiaojin,Wang Hongjiang,Zhang Lanlan,Wang Chunbo,Wang Wencheng,Liu Leqian,Wang Yuejun. 2009

[13]Polypeptide from Chlamys farreri inhibits UVB-induced apoptosis of HaCaT cells via iNOS/NO and HSP90. Zhang Zhengyang,Liu Xiaojin,Yan Lin,Wang Chunbo,Liu Tuo,Wang Yuejun. 2009

[14]Effects of nitrite exposure on haematological parameters, oxidative stress and apoptosis in juvenile turbot (Scophthalmus maximus). Jia, Rui,Huo, Huan-Huan,Jia, Rui,Lei, Ji-Lin,Liu, Bao-Liang,Huang, Bin,Yin, Shu-Ting,Han, Cen.

[15]Ability of Lactobacillus plantarum lipoteichoic acid to inhibit Vibrio anguillarum-induced inflammation and apoptosis in silvery pomfret (Pampus argenteus) intestinal epithelial cells. Gao, Quanxin,Min, Minghua,Zhang, Chenjie,Peng, Shiming,Shi, Zhaohong,Gao, Qian.

[16]Molecular cloning, immunohistochemical localization, characterization and expression analysis of caspase-8 from the blunt snout bream (Megalobrama amblycephala) exposed to ammonia. Sun, Shengming,Ge, Xianping,Zhu, Jian,Zhang, Wuxiao,Zhang, Qiong.

[17]Transcriptomic analysis of Mandarin fish brain cells infected with infectious spleen and kidney necrosis virus with an emphasis on retinoic acid-inducible gene 1-like receptors and apoptosis pathways. Hu, Xianqin,Dong, Xingxing,Zhao, Lijuan,Lan, Jiangfeng,Ji, Wei,Lin, Li,Fu, Xiaozhe,Li, Ningqiu,Wu, Shuqin,Hu, Xianqin,Fu, Xiaozhe,Li, Ningqiu,Wu, Shuqin,Lin, Li,Hu, Xianqin,Zhou, Weidong,Ai, Taoshan,Lin, Li.

[18]Immune and physiological responses of pufferfish (Takifugu obscurus) under cold stress. Cheng, Chang-Hong,Guo, Zhi-Xun,Ye, Chao-Xia,Wang, An-Li,Guo, Zhi-Xun.

[19]Effects of dietary resveratrol supplementation on hepatic and serum pro-/anti-inflammatory activity in juvenile GIFT tilapia, Oreochromis niloticus. Zheng, Yao,Wu, Wei,Song, Chao,Meng, Shunlong,Fan, Limin,Bing, Xuwen,Chen, Jiazhang,Zheng, Yao,Zhao, Zhixiang,Wu, Wei,Bing, Xuwen,Chen, Jiazhang.

[20]Construction of a mammalian cell expression vector pAcGFP-FasL and its expression in bovine follicular granulosa cells. Huang, Meng,Li, JunYa,Xu, ShangZhong,Yang, RunJun,Zhao, ZhiHui. 2011

作者其他论文 更多>>