Enolase-based nanovaccine immersion immunization induces robust immunity and protection against Streptococcus infection in tilapia
文献类型： 外文期刊
作者： Liu, Jia 1 ; Cao, Ye 1 ; Ma, Haixiang 1 ; Du, Hui 1 ; Liu, Tianqiang 1 ; Wang, Gaoxue 1 ; Liu, Mingzhu 4 ; Wang, Qing 2 ; Li, Pengfei 4 ; Wang, Erlong 1 ;
作者机构： 1.Northwest A&F Univ, Shenzhen Res Inst, Shenzhen 518000, Guangdong, Peoples R China
2.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Aquat Anim Immune Technol, Key Lab Fishery Drug Dev,Minist Agr & Rural Affair, Guangzhou 510380, Guangdong, Peoples R China
3.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China
4.Guangxi Acad Sci, Guangxi Acad Marine Sci, Guangxi Key Lab Aquat Biotechnol & Modern Ecol Aqu, Nanning, Peoples R China
关键词： Streptococcus; Enolase; Nanovaccine; Immunoprotective effect; Tilapia
期刊名称：AQUACULTURE （ 影响因子：4.5； 五年影响因子：4.6 ）
ISSN： 0044-8486
年卷期： 2023 年 576 卷
页码：
收录情况： SCI
摘要： Streptococcal disease is one of the most serious diseases in tilapia, causing huge economic losses to the tilapia farming industry. Streptococcus agalactiae (S. agalactiae) and Streptococcus iniae (S. iniae) are the two main pathogens of tilapia streptococcosis. Vaccination has been proved to be an effective measure to control fish bacterial diseases. In this study, we selected the Enolase (Eno) as the candidate vaccine antigen, which is the common antigen of S. agalactiae and S. iniae with high similarity (97.2%), the rEno was obtained and the CNTrEno nanocarrier vaccine system was constructed by connecting the rEno protein with functionalized carbon nanotubes (CNTs), the immersion immune effect against S. agalactiae and S. iniae infection was evaluated. The results showed that, compared with the control group, CNT-rEno significantly enhanced the serum antibody production, related enzyme activities and immune-related genes expression. It was worth noting that CNT-rEno demonstrated better immunoprotection against S. agalactiae and S. iniae infection, with relative percent survivals of 73.68% (S. agalactiae) and 68.96% (S. iniae), respectively, which were both much higher than those of rEno groups (36.84% and 31.03%, respectively). Our study indicated that the CNT-rEno nanovaccine had the ability to elicit robust immune responses in tilapia by immersion immunization, and had the potential to offer crossprotection against S. agalactiae and S. iniae infection.
- 相关文献
作者其他论文 更多>>
-
Application of microalgae in remediation of heavy metal-contaminated soils and its stimulatory effect on wheat growth
作者：Liu, Jia;Liu, Yajing;Jiang, Han;Yang, Xiaokun;Wu, Yukun;Liang, Chengwei;Zhang, Xiaowen;Ye, Naihao;Zhang, Xiaowen;Ye, Naihao
关键词：Microalgae; Heavy metal-contaminated soils; Bioremediation; Soil fertility; Wheat growth
-
Cyprinid herpesvirus 3 replication is inhibited via decreased ORF3 promoter activity mediated by multiple cellular transcription factors
作者：Liu, Donghai;Wang, Yingying;Li, Yingying;Mo, Xubing;Yin, Jiyuan;Wang, Qing;Zheng, Shucheng;Liu, Donghai
关键词：Cyprinid herpesvirus 3; Koi herpesvirus; ORF3; Promoter; Transcription factors
-
Enhanced virulence of Acinetobacter johnsonii at low temperatures induces acute immune response and systemic infection in American bullfrogs (Aquarana catesbeiana)
作者：Lin, Han;Sun, Jingyang;Li, Hua;Chen, Kesong;Qin, Zhendong;Jiang, Biao;Li, Wei;Su, Youlu;Lin, Li;Liu, Chun;Wang, Qing;Wang, Qing
关键词：Cold stress; Virulence genes; Amphibian disease; Immune response; Systemic infection
-
Dual impacts of elevated pCO2 on the ecological effects induced by microplastics and nanoplastics: A study with Chlamydomonas reinhardtii
作者：Liang, Chengwei;Liu, Yajing;Xi, Tianle;Liu, Jia;Jia, Zhihua;Liang, Chengwei;Ge, Shanshan;Zhang, Xiaowen;Ye, Naihao;Zhang, Xiaowen;Ye, Naihao
关键词：Micro-and nanoplastics; and nanoplastics; Freshwater acidification; Combined toxicity; Mechanisms; Oxidative stress; Chlamydomonas reinhardtii
-
Frog virus 3: Prevalence survey and quantitative analysis in American bullfrogs using TaqMan MGB probe real-time fluorescence quantitative PCR
作者：Li, Hua;Liu, Xiaobing;Sun, Jingyang;Lin, Han;Chen, Kesong;Hu, Yueming;Jiang, Mengzhen;Jiang, Biao;Li, Wei;Su, Youlu;Liu, Chun;Wang, Qing;Wang, Qing
关键词：Aquarana catesbeiana; Frog virus 3; TaqMan-MGB real-time PCR; Virus load; Prevalence survey
-
Enhanced stability and rheological properties of myofibrillar proteins emulsions conferred by oat β-glucan: Insights into structural and interfacial interactions
作者：Wu, Yanyan;Wu, Yamei;Zhao, Yongqiang;Xiang, Huan;Wang, Yueqi;Wu, Yanyan;Zhao, Yongqiang;Wang, Yueqi;Wu, Yamei;Hao, Zhiming;Wang, Qing
关键词：Oat beta-glucan; Myofibrillar protein; Conformational features; Interfacial property; Rheological property
-
Biological Characterization and Glucosinolate Degradation Mechanisms of Bacillus subtilis BSY82 in Rapeseed Meal
作者：Lu, Hong-yu;Shi, An-Ning;Liu, Li-Juan;Tang, Jiang-Yi;Wang, Ya-Jun;Zhang, De-Feng;Wang, Qing;Wang, Jun;Zhu, Xue-Zhi;Liu, Li-Yan
关键词：
Bacillus subtilis strain BSY82; biological characterization; fermented; glucosinolate; rapeseed meal
