Polymorphism in Growth Hormone Gene and its Association with Growth Traits in Siniperca chuatsi
文献类型: 外文期刊
作者: Wang, Haifang 1 ; Sun, Jijia 5 ; Wang, Pengfei 6 ; Lu, Xue; Xu, Peng 1 ; Gu, Yongming; Li, Guifeng 1 ;
作者机构: 1.Sun Yat Sen Univ, Inst Aquat Econ Anim, Guangzhou, Guangdong, Peoples R China
2.Sun Yat Sen Univ, South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China
3.Sun Yat Sen Univ, Sch Life Sci, Guangzhou, Guangdong, Peoples R China
4.Guangzhou Juyuan Biochem Co Ltd, Guangzhou, Guangdong, Peoples R China
5.South China Agr Univ, Coll Anim Sci, Guangzhou, Guangdong, Peoples R China
6.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources E
关键词: Siniperca chuatsi;Growth hormone;High-resolution melting;SNP;Growth traits
期刊名称:ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH ( 影响因子:0.287; 五年影响因子:0.593 )
ISSN: 0792-156X
年卷期: 2016 年 68 卷
页码:
收录情况: SCI
摘要: Growth hormone (GH) is a candidate gene for growth traits in fish. In this study, we assessed associations between single nucleotide polymorphisms (SNPs) in GH gene with growth traits in 357 Siniperca chuatsi individuals using high-resolution melting. Two SNPs were identified in GH gene, with one mutation in exon 5 (g.5045T>C), and one mutation in intron 5 (g.5234T>G). The corrections analysis of SNPs with the four growth traits was carried out using General Linear Model (GLM) estimation. Results showed that both of them were significantly associated with growth performance in S. chuatsi. For g. 5234T>G, it was significantly associated with body weight (P<0.01), body length (P<0.05), body depth (P<0.01), and body width (P<0.01), and the individuals of genotype GG grew faster than those of genotypes TT and TG (P<0.05). A further diplotype-trait association analysis confirmed that in fish with H3H2 (TC-GG) diplotype body weight, body length, and body width was greater than in those with other diplotypes (P< 0.05). These results demonstrated GH gene SNPs could be used as potential genetic markers in future marker assisted selection of S. chuatsi.
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