您好，欢迎访问内蒙古农牧业科学院 机构知识库！
内蒙古农牧业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Glutathione and selenium nanoparticles have a synergistic protective effect during cryopreservation of bull semen

文献类型： 外文期刊

作者： Li, Shubin 1 ; Ren, Jingyu 2 ; Zhang, Wenqi 1 ; Wang, Biao 3 ; Ma, Yuzhen 4 ; Su, Liya 1 ; Dai, Yanfeng 2 ; Liu, Gang 1 ;

作者机构： 1.Inner Mongolia Med Univ, Clin Med Res Ctr, Key Lab Med Cell Biol, Affiliated Hosp, Hohhot, Inner Mongolia, Peoples R China

2.Inner Mongolia Univ, Coll Life Sci, Hohhot, Inner Mongolia, Peoples R China

3.Anim Husb Inst, Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot, Inner Mongolia, Peoples R China

4.Inner Mongolia Peoples Hosp, Ctr Reprod Med, Hohhot, Inner Mongolia, Peoples R China

关键词： SeNPs; GSH; oxidative stress; bull; semen cryopreservation

期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：3.2； 五年影响因子：3.5 ）

ISSN：

年卷期： 2023 年 10 卷

页码：

收录情况： SCI

摘要： IntroductionIn the present study, the synergistic protective effect of co-supplementation of glutathione (GSH) with selenium nanoparticles (SeNPs) on the cryopreservation efficiency of bull semen was analyzed. MethodsAfter collection, the ejaculates of Holstein bulls were subsequently diluted with a Tris extender buffer supplemented with different concentrations of SeNPs (0, 1, 2, and 4 mu g/ml), followed by semen equilibration at 4 degrees C and assessment of sperm viability and motility. Subsequently, the ejaculates of Holstein bulls were pooled, split into four equal groups, and diluted with a Tris extender buffer supplemented with basic extender (negative control group, NC group), 2 mu g/ml SeNPs (SeNPs group), 4 mM GSH (GSH group), and 4 mM GSH plus 2 mu g/ml SeNPs (GSH + SeNPs group). After cryopreservation, motility, viability, mitochondrial activity, plasma membrane integrity, acrosome integrity, concentration of malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT), and ability of frozen-thawed sperm cells to support in vitro embryonic development were evaluated. Results and discussionNo side effect of SeNPs concentrations applied in the current study on the motility and viability of equilibrated bull spermatozoa was found. Meanwhile, supplementation of SeNPs significantly promoted the motility and viability of equilibrated bull spermatozoa. Furthermore, the co-supplementation of GSH with SeNPs effectively protected bull spermatozoa from cryoinjury as expressed by promoting semen motility, viability, mitochondrial activity, plasma membrane integrity, and acrosome integrity. Finally, the enhanced antioxidant capacity and embryonic development potential in the frozen-thawed bull spermatozoa cryopreserved by co-supplementation of GSH with SeNPs further confirmed the synergistic protective effect of co-supplementation of GSH with SeNPs on the cryopreservation of bull semen.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：内蒙古农牧业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台