Digital PCR-Based Characterization of a g10evo-epsps Gene-Specific Matrix Reference Material for Its Food and Feed Detection
文献类型： 外文期刊
作者： Chen, Xiaoyun 1 ; Yu, Huiru 2 ; Wang, Pengfei 3 ; Peng, Cheng 1 ; Wang, Xiaofu 1 ; Xu, Xiaoli 1 ; Xu, Junfeng 1 ; Liang, Jingang 4 ; Li, Liang 5 ;
作者机构： 1.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China
2.Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321001, Zhejiang, Peoples R China
3.Zhejiang Univ, Coll Agr & Biotechnol, Inst Insect Sci, Hangzhou 310058, Peoples R China
4.Minist Agr & Rural Affairs, Dev Ctr Sci & Technol, Beijing 100176, Peoples R China
5.Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Beijing 100081, Peoples R China
关键词： certified reference material; ZUTS-33 soybean; g10evo-epsps; digital PCR; glyphosate resistance
期刊名称：FOODS （ 影响因子：5.561； 五年影响因子：5.94 ）
ISSN：
年卷期： 2022 年 11 卷 13 期
页码：
收录情况： SCI
摘要： g10evo-epsps is a novel glyphosate herbicide-resistant gene that has been transferred to various crops such as soybean, corn, cotton, and rice. Here, we developed a gene-specific digital Polymerase Chain Reaction (dPCR) detection method for absolute quantitative analysis of g10evo-epsps, and characterized g10evo-epsps certified reference materials (CRM) using ZUTS-33 soybean powder as the candidate material. Stability tests of matrix CRMs demonstrate that these CRMs can be stored stably for 6 months and transported for 10 days at room temperature and withstand summer high temperatures (below 60 degrees C). CRM characterization is based on the copy number ratio of g10evo-epsps to lectin. Eight qualified laboratories independently validated the CRM using dPCR method, with a measurement of 0.98 (copy/copy) and an extended uncertainty of 0.08 (copy/copy). The g10evo-epsps matrix CRM described here may be used for qualitative and quantitative testing, method evaluation, laboratory quality control, and other related fields.
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