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Engineering Saccharomyces cerevisiae for improved biofilm formation and ethanol production in continuous fermentation

文献类型： 外文期刊

作者： Wang, Zhenyu 1 ; Xu, Weikai 1 ; Gao, Yixuan 1 ; Zha, Mingwei 1 ; Zhang, Di 1 ; Peng, Xiwei 1 ; Zhang, Huifang 1 ; Wang, Cheng 1 ; Xu, Chenchen 1 ; Zhou, Tingqiu 1 ; Liu, Dong 1 ; Niu, Huanqing 1 ; Liu, Qingguo 2 ; Chen, Yong 1 ; Zhu, Chenjie 1 ; Guo, Ting 3 ; Ying, Hanjie 1 ;

作者机构： 1.Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, State Key Lab Mat Oriented Chem Engn, Nanjing 211816, Peoples R China

2.Jiangsu Ind Technol Res Inst JITRI, Inst Ind Biotechnol, Nanjing 210032, Peoples R China

3.Jiangsu Acad Agr Sci, Nanjing 210014, Peoples R China

关键词： Biofilm; Ethanol; Continuous fermentation; Membrane separation

期刊名称：BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS

ISSN：

年卷期： 2023 年 16 卷 1 期

页码：

收录情况： SCI

摘要： Background Biofilm-immobilized continuous fermentation has the potential to enhance cellular environmental tolerance, maintain cell activity and improve production efficiency.Results In this study, different biofilm-forming genes (FLO5, FLO8 and FLO10) were integrated into the genome of S. cerevisiae for overexpression, while FLO5 and FLO10 gave the best results. The biofilm formation of the engineered strains 1308-FLO5 and 1308-FLO10 was improved by 31.3% and 58.7% compared to that of the WT strain, respectively. The counts of cells adhering onto the biofilm carrier were increased. Compared to free-cell fermentation, the average ethanol production of 1308, 1308-FLO5 and 1308-FLO10 was increased by 17.4%, 20.8% and 19.1% in the biofilm-immobilized continuous fermentation, respectively. Due to good adhering ability, the fermentation broth turbidity of 1308-FLO5 and 1308-FLO10 was decreased by 22.3% and 59.1% in the biofilm-immobilized fermentation, respectively. Subsequently, for biofilm-immobilized fermentation coupled with membrane separation, the engineered strain significantly reduced the pollution of cells onto the membrane and the membrane separation flux was increased by 36.3%.Conclusions In conclusion, enhanced biofilm-forming capability of S. cerevisiae could offer multiple benefits in ethanol fermentation.

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