您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

The CsNPR1 gene expression modulation in citrus and understanding the defense mechanism against Huanglongbing by screening CsNPR1-interacting proteins

文献类型： 外文期刊

作者： Wu, Qianqian 1 ; Moniruzzaman, M. 1 ; Yan, Huanxue 1 ; Lv, Yuanda 1 ; Jiang, Bo 1 ; Jiang, Nonghui 3 ; Zhong, Yun 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Guangzhou 510640, Peoples R China

2.Longchuan 1 High Sch, Heyuan 517300, Peoples R China

3.Minist Agr & Rural Affairs, Key Lab South Subtrop Fruit Biol & Genet Resource, Guangdong Prov Key Lab Trop & Subtrop Fruit Tree, Guangzhou 510640, Peoples R China

关键词： Citrus; CsNPR1; Stress resistance; Genetic transformation; Expression modulation; Interacting proteins

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN： 0304-4238

年卷期： 2021 年 288 卷

页码：

收录情况： SCI

摘要： Huanglongbing (HLB) disease, also known as citrus greening, is a severe threat for citrus fruit cultivation industry. Till date, neither any effective radical method to control HLB nor HLB resistant cultivar is developed. The aim of this study was to provide clues for unraveling the molecular regulatory mechanism of citrus in response to HLB, and to provide reference gene for breeding of HLB resistant cultivar. One-year-old plants of Shatian pomelo (Citrus grandis (L.) Osbeck) were treated with different hormones and adverse stresses. We found the disease resistance gene, CsNPR1, expression was modulated by gibberellin, indole-3-acetic acid, and salicylic acid, whereas significant expression changes also occurred under adverse stresses including drought, salt, and mechanical injury. Additionally, we enhanced the expression of CsNPR1 in citrus and evaluated plant resistance against HLB. Finally, to investigate CsNPR1 interacting proteins, a high-quality citrus cDNA library was constructed. The interacting proteins were screened and verified by yeast two-hybrid (Y2H) and Bimolecular Fluorescence Complementation (BiFC) assay. Two CsNPR1-interacting proteins (CsHSPRO2 and CsHIR2) were screened and verified. The results showed that the transgenic plants overexpressing CsNPR1 exhibited enhance tolerance against HLB. Thus, CsNPR1 plays a role in stress and disease defense in citrus through interaction with CsHSPRO2 or CsHIR2 proteins.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台