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Transciptomic and histological analysis of hepatopancreas, muscle and gill tissues of oriental river prawn (Macrobrachium nipponense) in response to chronic hypoxia

文献类型： 外文期刊

作者： Sun, Shengming 1 ; Xuan, Fujun 2 ; Fu, Hongtuo 1 ; Zhu, Jian 1 ; Ge, Xianping 1 ; Gu, Zhimin 3 ;

作者机构： 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Minist Agr, Key Lab Genet Breeding & Aquaculture Biol Freshwa, Wuxi 214081, Peoples R China

2.Jiangsu Prov Key Lab Coastal Wetland Bioresource, Yancheng City 224002, Jiangsu, Peoples R China

3.Zhejiang Inst Freshwater, Minist Agr, Key Lab Healthy Freshwater Aquaculture, Huzhou 313001, Peoples R China

关键词： Macrobrachium nipponense;RNA-Seq;Gene expression;Hypoxia

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2015 年 16 卷

页码：

收录情况： SCI

摘要： Background: Oriental river prawn, Macrobrachium nipponense, is a commercially important species found in brackish and fresh waters throughout China. Chronic hypoxia is a major physiological challenge for prawns in culture, and the hepatopancreas, muscle and gill tissues play important roles in adaptive processes. However, the effects of dissolved oxygen availability on gene expression and physiological functions of those tissues of prawns are unknown. Adaptation to hypoxia is a complex process, to help us understand stress-sensing mechanism and ultimately permit selection for hypoxia-tolerant prawns, we performed transcriptomic analysis of juvenile M. nipponense hepatopancreas, gill and muscle tissues by RNA-Seq. Results: Approximately 46,472,741; 52,773,612 and 58,195,908 raw sequence reads were generated from hepatopancreas, muscle and gill tissues, respectively. A total of 62,722 unigenes were generated, of the assembled unigenes, we identified 8,892 genes that were significantly up-regulated, while 5,760 genes were significantly down-regulated in response to chronic hypoxia. Genes from well known functional categories and signaling pathways associated with stress responses and adaptation to extreme environments were significantly enriched, including genes in the functional categories "response to stimulus", "transferase activity" and "oxidoreductase activity", and the signaling pathways "oxidative phosphorylation", "glycolysis/gluconeogenesis" and "MAPK signaling". The expression patterns of 18 DEGs involved in hypoxic regulation of M. nipponense were validated by quantitative real-time reverse-transcriptase polymerase chain reactions (qRT-PCR; average correlation coefficient = 0.94). In addition, the hepatopancreas and gills exhibited histological differences between hypoxia and normoxia groups. These structural alterations could affect the vital physiological functions of prawns in response to chronic hypoxia, which could adversely affect growth and survival of M. nipponense. Conclusions: Gene expression changes in tissues from the oriental river prawn provide a preliminary basis to better understand the molecular responses of M. nipponense to chronic hypoxia. The differentially expressed genes (DEGs) identified in M. nipponense under hypoxia stress may be important for future genetic improvement of cultivated prawns or other crustaceans through transgenic approaches aimed at increasing hypoxia tolerance.

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[1]Molecular Cloning and Functional Characterization of a Hexokinase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia. Sun, Shengming,Fu, Hongtuo,Zhu, Jian,Ge, Xianping,Xuan, Fujun. 2017

[2]Molecular characterization and mRNA expression of hypoxia inducible factor-1 and cognate inhibiting factor in Macrobrachium nipponense in response to hypoxia. Sun, Shengming,Fu, Hongtuo,Ge, Xianping,Zhu, Jian,Qiao, Hui,Jin, Shubo,Zhang, Wenyi,Xuan, Fujun.

[3]Identification of differentially expressed genes in hepatopancreas of oriental river prawn, Macrobrachium nipponense exposed to environmental hypoxia. Sun, Shengming,Ge, Xianping,Fu, Hongtuo,Zhu, Jian,Xuan, Fujun,Zhang, Shiyong.

[4]Identification and comparative analysis of the oriental river prawn (Macrobrachium nipponense) microRNA expression profile during hypoxia using a deep sequencing approach. Sun, Shengming,Fu, Hongtuo,Ge, Xianping,Zhu, Jian,Gu, Zhimin,Xuan, Fujun.

[5]Comparative proteomic study of the response to hypoxia in the muscle of oriental river prawn (Macrobrachium nipponense). Sun, Shengming,Fu, Hongtuo,Ge, Xianping,Zhu, Jian,Qiao, Hui,Jin, Shubo,Zhang, Yiwen,Xuan, Fujun.

[6]Molecular cloning, characterization, and expression analysis of p53 from the oriental river prawn, Macrobrachium nipponense, in response to hypoxia. Sun, Shengming,Fu, Hongtuo,Zhu, Jian,Ge, Xianping,Gu, Zhimin,Xuan, Fujun.

[7]THE METALLOTHIONEIN GENE FROM THE ORIENTAL RIVER PRAWN MACROBRACHIUM NIPPONENSE (DE HAAN, 1849): CHARACTERIZATION AND EXPRESSION IN RESPONSE TO HYPDXIA AND REOXYGENATION. Sun, Shengming,Gu, Zhimin,Fu, Hongtuo,Zhu, Jian.

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