Molecular characterization, expression following cold stress, and functional characterization of YB-1 gene in the spotted sea bass (Lateolabrax maculatus)
文献类型： 外文期刊
作者： Wang, Zhen 1 ; Wang, Jing 1 ; Mu, Weijie 1 ; Liang, Liqun 2 ;
作者机构： 1.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Harbin 150025, Peoples R China
2.Harbin Normal Univ, Coll Life Sci & Technol, Key Lab Biodivers Aquat Organisms, Harbin 150025, Peoples R China
关键词： YB-1; Lateolabrax maculatus; Cloning; Expression; Overexpression
期刊名称：AQUACULTURE INTERNATIONAL （ 影响因子：2.953； 五年影响因子：2.94 ）
ISSN： 0967-6120
年卷期：
页码：
收录情况： SCI
摘要： Existing data indicate that YB-1, a gene member of the cold chock domain family, is regulated by cold stress in many species. However, information regarding YB-1 in fish is limited. To gain more insight into the potential biological function of YB-1 after cold stress, we have cloned YB-1 of the spotted sea bass (Lateolabrax maculatus) and studied the expression at transcriptional level of the LmYB-1 gene under low temperature. The cloned LmYB-1 cDNA (1454 bp) includes a 900-bp open-reading frame that encodes a 300-residue protein and contains C-terminal, CSD, and N-terminal domains. The results of real-time quantitative PCR showed that LmYB-1 was expressed in all tested tissues, with the highest expression in liver. Within liver, brain, and heart, LmYB-1 mRNA expression increased during cold stressed fish, compared to control fish. No significant induces in the expression of LmYB-1 were recorded in muscle, except at 10 degrees C during 24 h cold stress. Overexpression of LmYB-1 in CIK cells caused the upregulation of the expression of Hsc70, SOD1, and SOD2. Our results provided new insights into the LmYB-1 expression during cold stress and warranted further investigation into this potentially important protein in improving fish farming at low temperature.
- 相关文献
作者其他论文 更多>>
-
Effects of Dietary Supplementation with Endogenous Probiotics Bacillus subtilis on Growth Performance, Immune Response and Intestinal Histomorphology of Juvenile Rainbow Trout (Oncorhynchus mykiss)
作者：Wang, Jing;Fan, Dan;Zhao, Ran;Lu, Tongyan;Li, Shaowu;Wang, Di
关键词：Oncorhynchus mykiss; Bacillus subtilis; growth; immunity; intestinal histology
-
Integrative analysis of transcriptomic profile reveals potential roles of miRNAs in regulating development of Marsupenaeus japonicas
作者：Wang, Jing;Pu, Longjun;Zhang, Xiaojuan;Liang, Cuicui;Dong, Dandan;Guo, Huarong;Guo, Huarong;Guan, Jiantao
关键词：microRNAs (miRNAs); identification; Marsupenaeus japonicus; embryo; transcriptome
-
Genomic insights into the seawater adaptation in Cyprinidae
作者：Wang, Ying;Zhang, Xuejing;Xiong, Fei;Zhou, Min;Wang, Jing;Wang, Cheng;Qian, Yuting;Meng, Minghui;Chen, Wenjun;Ding, Zufa;Yu, Dan;Liu, Yang;He, Shunping;Yang, Liandong;Wang, Ying;He, Shunping;Yang, Liandong;Wang, Cheng;Qian, Yuting;Chen, Wenjun;Ding, Zufa;Yu, Dan;Liu, Yang;Chang, Yumei;Wang, Ying;Yang, Liandong
关键词：Far Eastern dace; Migratory; Osmoregulation; Seawater adaptation
-
Isolation, Identification, and Characterisation of a Novel ST2378 Aeromonas hydrophila Strain from Naturally Diseased Frogs, Rana dybowskii
作者：Zhao, Ran;Wang, Jing;Wang, Di;Wang, Yanan;Hu, Guo;Li, Shaowu
关键词：Aeromonas hydrophila; Rana dybowskii; virulence genotype; antimicrobial susceptibility; MLST
-
Genome-Wide Identification, Molecular Characterization, and Involvement in Response to Abiotic and Biotic Stresses of the HSP70 Gene Family in Turbot (Scophthalmus maximus)
作者：Zheng, Weiwei;Xu, Xiwen;Chen, Yadong;Zhang, Tingting;Zechen, E.;Chen, Songlin;Zheng, Weiwei;Chen, Yadong;Wang, Jing;Zhang, Tingting;Zechen, E.;Chen, Songlin;Liu, Yingjie;Xu, Xiwen;Chen, Yadong;Chen, Songlin;Liu, Yingjie
关键词：Scophthalmus maximus; heat shock protein 70 (HSP70); abiotic stress; biotic stress
-
A novel miRNA, Cse-miR-33, functions as an immune regulator by targeting CsTRAF6 in Chinese tongue sole (Cynoglossus semilaevis)
作者：Li, Xihong;Chen, Yadong;Lin, Mengjiao;Wang, Na;Chen, Zhangfan;Chen, Songlin;Li, Xihong;Chen, Yadong;Lin, Mengjiao;Wang, Na;Chen, Zhangfan;Chen, Songlin;Li, Xihong;Chen, Yadong;Wang, Na;Chen, Zhangfan;Chen, Songlin;Lin, Mengjiao;Wang, Jing
关键词：Cynoglossus semilaevis; miR-33; TRAF6; Negative regulation; Immune effect
-
Comparison of macrofaunal assemblages in temperate seagrass meadows and neighboring seabeds along the southeastern coast of Shandong Peninsula, China
作者：Bi, Peiru;Liu, Xiaoshou;Bi, Peiru;Liu, Xiaoshou;Bi, Peiru;Liu, Xiaoshou;Chen, Guangcheng;An, Wenshuo;Chen, Guangcheng;An, Wenshuo;Wang, Jiming;Hu, Hongzhi;Jiang, Zengjie;Zhang, Jing;Wang, Jing;Liu, Xiaoshou;Liu, Xiaoshou
关键词：Seagrass meadow; Macrofauna; Community structure; Species diversity; Shandong Peninsula
