文献类型： 外文期刊

作者： Wang, Ying ¹ ; Zhang, Xuejing ¹ ; Wang, Jing ² ; Wang, Cheng ² ; Xiong, Fei ¹ ; Qian, Yuting ² ; Meng, Minghui ² ; Zhou, Min ¹ ; Chen, Wenjun ² ; Ding, Zufa ² ; Yu, Dan ² ; Liu, Yang ² ; Chang, Yumei ⁵ ; He, Shunping ² ; Yang, Liandong ² ;

作者机构： 1.Jianghan Univ, Coll Life Sci, Hubei Engn Res Ctr Protect & Utilizat Special Biol, Wuhan 430056, Peoples R China

2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

3.Qinghai Normal Univ, Acad Plateau Sci & Sustainabil, Xining 810016, Peoples R China

4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

5.Chinese Acad Fishery Sci, Natl & Local Joint Engn Lab Freshwater Fish Breedi, Heilongjiang Prov Key Lab Fish Stress Resistance B, Heilongjiang River Fisheries Res Inst, Harbin 150070, Heilongjiang, Peoples R China

6.Univ Bristol, Sch Biol Sci, Life Sci Bldg,24 Tyndall Ave, Bristol BS8 1TQ, England

关键词： Far Eastern dace; Migratory; Osmoregulation; Seawater adaptation

期刊名称：BMC BIOLOGY （ 影响因子：5.4； 五年影响因子：7.1 ）

ISSN：

年卷期： 2024 年 22 卷 1 期

页码：

收录情况： SCI

摘要： Background Cyprinidae, the largest fish family, encompasses approximately 367 genera and 3006 species. While they exhibit remarkable adaptability to diverse aquatic environments, it is exceptionally rare to find them in seawater, with the Far Eastern daces being of few exceptions. Therefore, the Far Eastern daces serve as a valuable model for studying the genetic mechanisms underlying seawater adaptation in Cyprinidae.Results Here, we sequenced the chromosome-level genomes of two Far Eastern daces (Pseudaspius brandtii and P. hakonensis), the two known cyprinid fishes found in seawater, and performed comparative genomic analyses to investigate their genetic mechanism of seawater adaptation. Demographic history reconstruction of the two species reveals that their population dynamics are correlated with the glacial-interglacial cycles and sea level changes. Genomic analyses identified Pseudaspius-specific genetic innovations related to seawater adaptation, including positively selected genes, rapidly evolving genes, and conserved non-coding elements (CNEs). Functional assays of Pseudaspius-specific variants of the prolactin (prl) gene showed enhanced cell adaptation to greater osmolarity. Functional assays of Pseudaspius specific CNEs near atg7 and usp45 genes suggest that they exhibit higher promoter activity and significantly induced at high osmolarity.Conclusions Our results reveal the genome-wide evidence for the evolutionary adaptation of cyprinid fishes to seawater, offering valuable insights into the molecular mechanisms supporting the survival of migratory fish in marine environments. These findings are significant as they contribute to our understanding of how cyprinid fishes navigate and thrive in diverse aquatic habitats, providing useful implications for the conservation and management of marine ecosystems.