您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Separation, Purification, and Identification of (3S,3 ' S)-trans-Astaxanthin from Haematococcus pluvialis

文献类型： 外文期刊

作者： Sun, Weihong 1 ; Lin, Hong 1 ; Zhai, Yuxiu 2 ; Cao, Limin 1 ; Leng, Kailiang 2 ; Xing, Lihong 2 ;

作者机构： 1.Ocean Univ China, Dept Food Sci & Engn, Qingdao 266100, Shandong, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fishery Res Inst, Qingdao, Shandong, Peoples R China

关键词： identification;Haematococcus pluvialis;separation;(3S,3 ' S)-trans-astaxanthin;purification

期刊名称：SEPARATION SCIENCE AND TECHNOLOGY （ 影响因子：2.475； 五年影响因子：2.077 ）

ISSN： 0149-6395

年卷期： 2015 年 50 卷 9 期

页码：

收录情况： SCI

摘要： A simple, low-cost method of separation and purification for preparing (3S,3 ' S)-trans-astaxanthin from Haematococcus pluvialis was established in this study. Crude extracts were separated from dry algal cells by extraction. The extracts were then saponified at 4 degrees C for 15 h and at 22 degrees C for 3 h with 0.02 M NaOH in the reaction mixture with lower isomerization of trans-astaxanthin to cis isomers. The saponified pigment extracts were mixed with distilled water and n-hexane at 1:1:1 volume ratio. Low-pressure column chromatography of silica gel (300-400 mesh) with different eluents was used to prepare high-purity (> 80%) all-trans-astaxanthin. Supersaturated solution of astaxanthin in acetone was then crystallized for 72 h at 4 degrees C, and the purity of all-trans-astaxanthin crystalline powder was confirmed to be > 96.5% by HPLC. And the 3S,3 ' S isomers were identified and characterized by HPLC-APCI-MS, H-1 NMR, C-13 NMR, and HPLC-UV.

  • 相关文献

[1]Analysis of mRNA expression profiles of carotenogenesis and astaxanthin production of Haematococcus pluvialis under exogenous 2, 4-epibrassinolide (EBR). Gao, Zhengquan,Meng, Chunxiao,Gao, Hongzheng,Su, Yuanfeng,Wang, Yuanyuan,Zhao, Yuren,Zhang, Xiaowen,Xu, Dong,Ye, Naihao. 2013

[2]Correlation between lipid and carotenoid synthesis and photosynthetic capacity in Haematococcus pluvialis grown under high light and nitrogen deprivation stress. Liang, C.,Zhai, Y.,Zhang, W.,Yu, J.,Xu, D.,Ye, N.,Zhang, X.,Wang, Y.. 2015

[3]The induction of ferrous sulfate or sodium acetate on mRNA expression of carotenoid genes in Haematococcus pluvialis. Gao Zhengquan,Meng Chunxiao,Gao Hongzheng,Su Yuanfeng,Zhang Xiaowen,Ye Naihao. 2013

[4]Carotenoid genes transcriptional regulation for astaxanthin accumulation in fresh water unicellular alga Haematococcus pluvialis by gibberellin A3 (GA(3)). Gao, Zhengquan,Meng, Chunxiao,Gao, Hongzheng,Zhou, Shitan,Liu, Banghui,Su, Yuanfeng,Li, Yan,Zhang, Xiaowen,Xu, Dong,Ye, Naihao. 2013

[5]Isolation and expression analyses of methyl-d-erythritol 4-phosphate (MEP) pathway genes from Haematococcus pluvialis. Liang, Chengwei,Zhang, Wei,Su, Zhongliang,Yu, Jiansheng,Zhang, Xiaowen,Fan, Xiao,Xu, Dong,Ye, Naihao,Yang, Qingli.

[6]Effect of Exogenous Ethylene (ET) on Regulating Carotenogenesis Expression and Astaxanthin Accumulation in Haematococcus pluvialis. Gao, Zhengquan,Meng, Chunxiao,Gao, Hongzheng,Su, Yuanfeng,Zhao, Yuren,Wang, Yuanyuan,Ye, Naihao,Zhang, Xiaowen.

[7]Development of A Monoclonal Antibody-Coated Immunomagnetic Beads for Separation and Detection of Norovirus (Genogroup II) in Oysters. Yao, Lin,Jiang, Yanhua,Jiang, Wei,Li, Fengling,Zhai, Yuxiu,Wang, Lianzhu. 2013

[8]Identification of Symbiotic or Epiphyte Microbe F17 from Pinctada Fucata and Analysis of Its Main Metabolites by GC-MS. Yanyan Wu,Yan Zhang,Laihao Li,Xianqing Yang,Wei Shi,Zhengyi Shao. 2014

[9]Isolation and Identification of Nitrite-degrading Lactic Acid Bacteria from Salted Fish. Yanyan Wu,Fajia Liu,Laihao Li,Xianqing Yang,Jianchao Deng,Sheng-jun Chen. 2012

[10]Purification and properties of lysozyme from a marine strain. Zhang, Xiu,Ren, Xian,Zhang, Xiu,Wang, Yue-Jun,Sun, Mi,Zou, Yan-Li,Wang, Qing-Yin,Wang, Wei. 2008

[11]Extraction, Purification and partial characterizations of Polysaccharides from Gracilaria lemaneiformis. Yang, Xianqing,Liu, Mingqiu,Qi, Bo,Li, Laihao,Deng, Jianchao,Hu, Xiao,Wu, Yanyan,Hao, Shuxian. 2014

[12]Purification and characterization of a psychrophilic catalase from Antarctic Bacillus. Wang, Wei,Sun, Mi,Zhang, Bin,Liu, Wanshun. 2008

[13]Purification and characteristics of serine protease from the head of pacific white shrimp. Wu, Yanyan,Li, Laihao,Yang, Xianqing,Diao, Shiqiang,Wang, Ping. 2012

[14]Purification of antimicrobial peptide from Antarctic Krill (Euphausia superba) and its function mechanism. Zhao Ling,Yin Bangzhong,Liu Qi,Cao Rong. 2013

[15]Production and Characterization of a New alpha-Cyclodextrin Glycosyltransferase from a Marine Strain of Bacillus sp Y112. Sun, Jing-Jing,Liu, Jun-Zhong,Wang, Wei,Sun, Mi,Hao, Jian-Hua,Sun, Jing-Jing,Liu, Jun-Zhong,Wang, Wei,Sun, Mi,Hao, Jian-Hua,Chen, Xiao-Tong,Huang, Li-Ping. 2017

[16]Effects of physicochemical factors and in vitro gastrointestinal digestion on antioxidant activity of R-phycoerythrin from red algae Bangia fusco-purpurea. Wu, Qiang,Fu, Xiao-Ping,Sun, Le-Chang,Liu, Guang-Ming,Cao, Min-Jie,Cai, Qiu-Feng,Fu, Xiao-Ping,Zhang, Qian,Liu, Guang-Ming,Cao, Min-Jie,Cai, Qiu-Feng,Liu, Guang-Ming,Cao, Min-Jie,Cai, Qiu-Feng.

[17]Optimization of the purification methods for recovery of recombinant growth hormone from Paralichthys olivaceus. Liu Bin,Zang Xiaonan,Zhang Xuecheng,Mu Xiaosheng. 2013

[18]Expression, purification and characterization of WSSV-VP37 in Pichia pastoris. Liu Qing-hui,Huang Jie,Han Wen-jun,Liang Yan,Lu Chun-ling,Wang Qing-yin. 2006

[19]Reovirus infection emerged in cultured channel catfish, Ictalurus punctatus, in China. Zeng, Lingbing,Luo, Xiaosong,Fan, Yuding,Xu, Jin,Wang, Yao,Gong, Shiyuan. 2013

[20]Isolation, Identification, and Detection, of the Virulence Factor of Vibrio cholerae in Grass Carp. Teng, Tao,Liang, Liguo,Xie, Jun. 2015

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台