Vibrio parahaemolyticus becomes lethal to post-larvae shrimp via acquiring novel virulence factors
文献类型: 外文期刊
作者: Liu, Shuang 1 ; Wang, Wei 1 ; Jia, Tianchang 1 ; Xin, Lusheng 1 ; Xu, Ting-ting 1 ; Wang, Chong 1 ; Xie, Guosi 1 ; Luo, Kun 1 ; Li, Jun 4 ; Kong, Jie 1 ; Zhang, Qingli 1 ;
作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Qingdao, Shandong, Peoples R China
2.Laoshan Lab, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Shandong, Peoples R China
3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Marine Aquaculture Dis Control, Key Lab Sustainable Dev Marine Fisheries, Qingdao, Shandong, Peoples R China
4.Lake Super State Univ, Sch Sci & Med, Sault Ste Marie, ND USA
关键词: translucent post-larvae disease (TPD); Vibrio parahaemolyticus-causing TPD (Vp (TPD)); shrimp; novel virulence factor; Vibrio high virulent protein (VHVP)
期刊名称:MICROBIOLOGY SPECTRUM ( 影响因子:3.7; 五年影响因子:5.9 )
ISSN: 2165-0497
年卷期: 2023 年
页码:
收录情况: SCI
摘要: Translucent post-larvae disease (TPD), caused by Vibrio parahaemolyticus (Vp (TPD)), has become an emerging shrimp disease, affecting more than 70%-80% of coastal shrimp nurseries in China in spring 2020. Here, we investigated the key virulence factors of Vp (TPD) by analyzing protein fragments, related genomic information, as well as experimental challenge tests. After investigating the toxic effects of purified protein fragments with different molecular weights (MWs) from Vp (TPD,) we found that only the protein fragments with MW >100 kDa showed similar lethality to live Vp (TPD) in the experimental challenge test using post-larvae shrimp. Meanwhile, similar histopathological changes exhibiting in the hepatopancreas and midgut of the diseased individuals were observed in the post-larvae shrimp challenged with either bacterial protein fragments (MW >100 kDa) or live Vp (TPD). Based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometry analyses, two novel proteins, Vibrio high virulent protein (VHVP)-1 and VHVP-2, were identified as the candidates of key virulence factors to cause TPD. Moreover, VHVP-1 and VHVP-2 were found to be encoded by two genes (vhvp-1 and vhvp-2) tandemly located on a 187,791-bp plasmid and were predicted to depend on the same promoter following a comparative genomic analysis. Further epidemiological investigation and challenge test indicated that the V. parahaemolyticus isolate carrying only the vhvp-1 gene and lacking vhvp-2 gene could not cause mortality of experimental Penaeus vannamei post-larvae. The mutant (Delta vhvp-2) by deleting vhvp-2 gene could only cause 4.92% of accumulative mortality of post-larvae that is similar to the non-Vp (TPD) Vibrio strain. Additionally, the complemented strains, Delta vhvp-2/pBT3-vhvp-2 and Delta vhvp-2/pwtCas9-vhvp-2, showed similar virulence to the wild-type Vp (TPD). The results demonstrated that V. parahaemolyticus becomes lethal to post-larval shrimp by acquiring the VHVP-2 virulence factor. This study sheds light on further investigations of the pathogenic mechanism of Vp (TPD) and the development of strategies for early diagnosis of TPD in shrimp hatcheries.
- 相关文献
作者其他论文 更多>>
-
A rapid one-step affinity purification of C-phycocyanin from Spirulina platensis
作者:Shi, Ke;Shi, Ke;Wang, Wei;Sun, Jingjing;Jiang, Chengcheng;Hao, Jianhua;Hao, Jianhua
关键词:C-phycocyanin; Spirulina platensis; Affinity chromatography; Virtual screening
-
Analysis of Elimination Effects of Inbreeding on Genotype Frequency in Larval Stages of Chinese Shrimp
作者:Fu, Qiang;Zhou, Jingxin;Luan, Sheng;Dai, Ping;Lyu, Ding;Chen, Baolong;Luo, Kun;Kong, Jie;Meng, Xianhong;Fu, Qiang;Zhou, Jingxin;Luan, Sheng;Dai, Ping;Lyu, Ding;Chen, Baolong;Luo, Kun;Kong, Jie;Meng, Xianhong
关键词:Fenneropenaeus chinensis; inbreeding; larval development; segregation distortion; SNPs
-
Comparative Transcriptomic Analysis of WSSV-Challenged Penaeus vannamei with Variable Resistance Levels
作者:Li, Xupeng;Xue, Qian;Luan, Sheng;Luo, Kun;Kong, Jie;Meng, Xianhong;Li, Xupeng;Luan, Sheng;Kong, Jie;Meng, Xianhong
关键词:WSSV; Penaeus vannamei; immunity; family; resistant ability; viral load; transcriptome
-
Biochemical Characterization of a Heat-Resistant κ-Carrageenase Capable of Tolerating High Temperatures up to 100 °C
作者:Jiang, Chengcheng;Wang, Wei;Sun, Jingjing;Hao, Jianhua;Jiang, Chengcheng;Wang, Wei;Sun, Jingjing;Hao, Jianhua;Mao, Xiangzhao;Hao, Jianhua
关键词:carrageenan; kappa-carrageenase; noncatalyticdomain; enzymatic properties
-
Quantifying the spatial nonstationary response of environmental factors on purse seine tuna vessel fishing
作者:Wang, Wei;Zhang, Shengmao;Wang, Wei;Fan, Wei;Wu, Yumei;Zhang, Shengmao;Zhou, Weifeng;Fan, Xiumei;Yang, Shenglong;Wang, Wei;Fan, Wei;Wu, Yumei;Zhang, Shengmao;Zhou, Weifeng;Fan, Xiumei;Yang, Shenglong;Shi, Jiashu;Jin, Weiguo;Wang, Guolai
关键词:Tuna; Western and Central Pacific Ocean; Fishing effort; GWR; Spatial distribution; Spatial heterogeneity
-
Systematic review on carrageenolytic enzymes: From metabolic pathways to applications in biotechnology
作者:Jiang, Chengcheng;Ma, Yuqi;Wang, Wei;Sun, Jingjing;Hao, Jianhua;Ma, Yuqi;Mao, Xiangzhao;Hao, Jianhua;Hao, Jianhua
关键词:Carrageenan; Carrageenolytic enzymes; Enzymatic characteristics; Metabolic pathways
-
A Novel Transposon Tn7709 Harbors Multidrug Resistance Genes in a Pathogenic Aeromonas media Strain QST31
作者:Shang, Baodi;Li, Xiaoyi;Zhang, Xiaoping;Zhang, Meiyan;Kong, Jie;Wang, Jinle;Zhao, Feng;Tan, Aiping;Zhang, Defeng;Tan, Aiping;Zhang, Defeng
关键词:Aeromonas media; antimicrobial resistance genes; transposon; integron; tadpoles