Molecular identification and efficacy assessment of a glufosinate-tolerant and brown planthopper-resistant transgenic rice line
文献类型： 外文期刊
作者： Li, Sanhe 1 ; Zha, Wenjun 1 ; Liu, Kai 1 ; Li, Chen 2 ; Zhou, Lei 1 ; He, Guangcun 3 ; Xu, Huashan 1 ; Li, Peide 1 ; Chen, Junxiao 1 ; Chen, Zhijun 1 ; You, Aiqing 1 ;
作者机构： 1.Hubei Acad Agr Sci, Food Crops Inst, Hubei Key Lab Food Crop Germplasm & Genet Improve, Wuhan, Peoples R China
2.Wuhan Lichen Biotechnol Co Ltd, Wuhan, Peoples R China
3.Wuhan Univ, State Key Lab Hybrid Rice, Wuhan, Peoples R China
关键词： Brown planthopper; Glufosinate; H23; Field trial; Transgenic rice
期刊名称：JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：3.686； 五年影响因子：4.352 ）
ISSN： 0176-1617
年卷期： 2022 年 273 卷
页码：
收录情况： SCI
摘要： Insect pests and weeds are the two major biotic factors affecting crop yield in the modern agricultural system. In this study, a brown planthopper (BPH) resistance gene (BPH9) and glufosinate tolerance gene (bar) were stacked into a single T-DNA cassette and transformed into an indica rice (Oryza sativa L.) line Guangzhan 63-4S. A stable transgenic line H23 with a single T-DNA insert was generated, with the T-DNA cassette located on chromosome 3. Field resistance trial using H23 revealed high tolerance to glufosinate and excellent resistance to BPH. These results propose H23 as valuable germplasm for BPH-resistance and glufosinate-tolerance breeding in rice.
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