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RIPK3-Dependent Necroptosis Limits PRV Replication in PK-15 Cells

文献类型： 外文期刊

作者： Gou, Hongchao 1 ; Bian, Zhibiao 1 ; Cai, Rujian 1 ; Chu, Pinpin 1 ; Song, Shuai 1 ; Li, Yan 1 ; Jiang, Zhiyong 1 ; Zhang, 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou, Peoples R China

2.Guangdong Prov Key Lab Livestock Dis Prevent, Guangzhou, Peoples R China

3.Guangdong Lab Lingnan Modern Agr, Maoming Branch, Maoming, Peoples R China

4.Sci Observat & Expt Stn Vet Drugs & Diagnost Tech, Guangzhou, Peoples R China

关键词： pseudorabies virus (PRV); necroptosis; receptor interacting protein kinase 3 (RIPK3); mixed lineage kinase-like protein (MLKL); PK-15 cell

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2021 年 12 卷

页码：

收录情况： SCI

摘要： Pigs infected by pseudorabies virus (PRV) display necrotic pathology in multiple organs. The mechanism by which PRV induces cell death is still unclear. Recently, necroptosis was identified as a programmed process dependent on the receptor interacting protein kinase 3 (RIPK3) and mixed lineage kinase-like protein (MLKL). In this study, we demonstrated that PRV induced RIPK3-dependent necroptosis in PK-15 cells. The data showed that PRV infection caused cell death with Propidium Iodide (PI)-positive staining. Transmission electron microscopy analysis indicated plasma membrane disruption in PRV-infected cells. A pan-caspase inhibitor did not prevent PRV-induced necrotic cell death. Western blot analysis indicated that caspase-3 and caspase-8 were not cleaved during PRV infection. Although the transcription of tumor necrosis factor-alpha (TNF-alpha) was increased by PRV infection, RIPK1 was shown to be not involved in PRV-induced necrotic cell death by use of its specific inhibitor. Further experiments indicated that the phosphorylation of RIPK3 and MLKL was upregulated in PRV-infected cells. Stable shRNA knockdown of RIPK3 or MLKL had a recovery effect on PRV-induced necrotic cell death. Meanwhile, viral titers were enhanced in RIPK3 and MLKL knockdown cells. Hence, we concluded that initiation of necroptosis in host cells plays a limiting role in PRV infection. Considering that necroptosis is an inflammatory form of programmed cell death, our data may be beneficial for understanding the necrotic pathology of pigs infected by PRV.

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