FIP-fve Stimulates Cell Proliferation and Enhances IL-2 Release by Activating MAP2K3/p38 alpha (MAPK14) Signaling Pathway in Jurkat E6-1 Cells
文献类型： 外文期刊
作者： Gu, Kefei 1 ; Wang, Tan 1 ; Peng, Liying 4 ; Zhao, Yueliang 2 ;
作者机构： 1.Inst Agrifood Stand & Testing Technol, Shanghai Acad Agr Sci, Shanghai, Peoples R China
2.Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai, Peoples R China
3.Minist Agr, Lab Qual & SafetyRisk Assessment Aquat Prod Storag, Shanghai, Peoples R China
4.Inst Anim Husb & Vet Sci, Shanghai Acad Agr Sci, Shanghai, Peoples R China
关键词： FIP-fve; immunomodulation mechanism; proteomics analysis; MAP2K3/p38 alpha; IL-2
期刊名称：FRONTIERS IN NUTRITION （ 影响因子：6.59； 五年影响因子：6.873 ）
ISSN： 2296-861X
年卷期： 2022 年 9 卷
页码：
收录情况： SCI
摘要： FIP-fve, a fungal fruiting body protein from Flammulina velutipes, has potential immunomodulatory properties. Here, we investigated the immunomodulation mechanism of FIP-fve in Jurkat E6-1 cells by conducting a cell viability assay and IL-2 release assay. Kinase inhibitors experiment and proteomics analysis were also involved in the mechanism study. It was found that FIP-fve stimulated cell proliferation and enhanced IL-2 secretion in a dose-dependent manner in Jurkat E6-1 cells. Unbiased high-throughput proteomics analysis showed that 4 T cell immune activation markers, including ZAP-70, CD69, CD82, and KIF23, were upregulated in response to FIP-fve treatment. Further pathway analysis indicated that MAP2K3/p38 pathway-related proteins, including MAP2K, p38, ELK, AATF, FOS, and JUN-B, were unregulated. In addition, losmapimod (p38 inhibitor) and gossypetin (MAP2K3 inhibitor) inhibited FIP-fve enhanced cell proliferation and IL-2 release in Jurkat E6-1 cells. Our results demonstrate that FIP-fve stimulates cell proliferation and enhances IL-2 secretion through MAP2K3/p38 alpha activation.
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