Identification, expression, and characterization of a marine-derived chitinase Ce 0303 from Chitiniphilus eburneus YS-30 with exo- and endo- hydrolytic properties
文献类型： 外文期刊
作者： Yu, Yuan 1 ; Chen, Sunan 2 ; Yan, Mingyan 2 ; Li, Yinping 2 ; Yang, Min 3 ; Liu, Xiaofang 1 ; Miao, Junkui 1 ; Wang, Xixi 1 ; Xiao, Mengshi 3 ; Mou, Haijin 3 ; Leng, Kailiang 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fishery, Minist Agr & Rural Affairs, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Qingdao 266042, Shandong, Peoples R China
3.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266100, Shandong, Peoples R China
4.Qingdao Marine Sci & Technol Ctr, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China
5.Ocean Univ China, Coll Informat Sci & Engn, 238 Songling Rd, Qingdao 266100, Shandong, Peoples R China
6.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, 106 Nanjing Rd, Qingdao 266071, Shandong, Peoples R China
关键词： Chitinase; N -acetyl-oligosaccharides; Crystalline chitin
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：7.7； 五年影响因子：7.7 ）
ISSN： 0141-8130
年卷期： 2024 年 276 卷
页码：
收录情况： SCI
摘要： N-acetyl-oligosaccharides exhibit antioxidant and antibacterial activities. However, the low catalytic efficiency of chitinase on crystalline chitin hinders the eco-friendly production of N-acetyl-oligosaccharides. A marinederived chitinase-producing strain Chitiniphilus eburneus YS-30 was screened in this study. The genome of C. eburneus YS-30 spans 4,522,240 bp, with a G + C content of 63.96 % and 4244 coding genes. Among the chitinases secreted by C. eburneus YS-30, Ce0303 showed the highest content at 19.10 %, with a molecular weight of 73.5 kDa. Recombinant Ce0303 exhibited optimal activity at 50 degrees C and pH 5.0, maintaining stability across pH 4.0-10.0. Ce0303 demonstrated strict substrate specificity, with a specific activity toward colloidal chitin of 6.41 U mg-1, Km of 2.34 mg mL-1, and kcat of 3.27 s-1. The specific activity of Ce0303 toward alpha-chitin was 18.87 % of its activity on colloidal chitin. Ce0303 displayed both exo- and endo-hydrolytic properties, primarily producing (GlcNAc)1-3 from colloidal chitin. The structure of Ce0303 includes one catalytic domain and two chitinbinding domains. Docking results revealed that the GlcNAc at -1 subsite formed two hydrogen bonds with conserved Trp380. The hydrolytic properties of Ce0303 will provide technical support for the comprehensive utilization of crustacean raw materials.
- 相关文献
作者其他论文 更多>>
-
Computer-Aided Rational Design Strategy to Improve the Thermal Stability of Alginate Lyase AlyMc
作者：Cui, Yongyan;Cui, Yongyan;Yang, Min;Liu, Nan;Wang, Shanshan;Sun, Yong;Sun, Guohui;Zhou, Deqing;Yang, Min;Mou, Haijin
关键词：alginate lyase; alginate oligosaccharides; rational design; thermal stability; moleculardynamicssimulation
-
A comprehensive study of the colloidal properties, biocompatibility, and synergistic antioxidant actions of Antarctic krill phospholipids
作者：Su, Dong;Zhang, Zipeng;Zhang, Yating;Zhao, Ling;Yu, Yueqin;Su, Dong;Wang, Xixi;Liu, Xiaofang;Miao, Junkui;Yu, Yuan;Leng, Kailiang;Leng, Kailiang;Leng, Kailiang
关键词：Antarctic krill; PUFAs-esterified phospholipid; Antioxidant; Synergy; Oxygen-sensitive
-
Comparative metabolomics study on the quality of Antarctic krill (Euphausia superba) stored at different temperatures
作者：Ma, Xinyu;Su, Dong;Xie, Wancui;Ma, Xinyu;Su, Dong;Zhu, Jiancheng;Miao, Junkui;Liu, Xiaofang;Leng, Kailiang;Wang, Xixi;Leng, Kailiang
关键词：Antarctic krill; metabolomics; quality biomarkers; short-term storage; temperature variation
-
Transcriptome response of Antarctic Phaeodactylum tricornutum ICE-H producing dimethylsulphoniopropionate to hypersaline stress
作者：Wang, Xixi;Miao, Junkui;Liu, Xiaofang;Yu, Yuan;Leng, Kailiang;Qu, Changfeng;Miao, Jinlai;Qu, Changfeng;Miao, Junkui;Liu, Xiaofang;Yu, Yuan;Leng, Kailiang;Miao, Jinlai;Leng, Kailiang;Miao, Jinlai
关键词：Antarctic P; tricornutum ICE -H; Dimethylsulfoniopropionate; Hypersaline stress; Transcriptome profiling
-
Krill oil treatment ameliorates lipid metabolism imbalance in chronic unpredicted mild stress-induced depression-like behavior in mice
作者：Zhang, Hao;Li, Bo;Zhang, Yi;Gao, Hua;Song, Zhenhua;Liu, Xiaofang;Zhao, Xianyong;Leng, Kailiang
关键词：major depressive disorder; CUMS; krill oil; transcriptome sequencing; lipid metabolism
-
High-hydrostatic-pressure inactivation of GI.5 and GII.4 human norovirus and effects on the physical, chemical, and taste characteristics of oyster (Crassostrea virginica)
作者：Ding, Guoying;Zhao, Feng;Tong, Lihui;Yang, Min;Wang, Shanshan;Zhou, Deqing;Tong, Lihui;Su, Laijin;Wang, Yanbo;Zheng, Lei
关键词：Human norovirus; Oyster; High hydrostatic pressure; Inactivation; Quality
-
Antibacterial activity of bifunctional bacterial cellulose composite grafted with glucose oxidase and l-arginine
作者：Zhao, Bingxin;Yuan, Mingxue;Liu, Zhemin;Mukhtar, Hina;Zhu, Changliang;Sun, Han;Mou, Haijin;Wang, Lianzhu;Fu, Xiaodan;Yao, Min
关键词：Bacterial cellulose; Glucose oxidase; Antibacterial; l-arginine; Schiff base
