文献类型： 外文期刊
作者： He, Yijing 1 ; Li, Jia 1 ; Peng, Lei 1 ; Li, Qiao 1 ; Chu, Yajie 1 ; Lin, Qixin 1 ; Dai, Jianjun 2 ; Rui, Rong 1 ; Ju, Shiqiang 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Vet Med, MOE Joint Int Res Lab Anim Hlth & Food Safety, 1 Weigang, Nanjing 210095, Peoples R China
2.Shanghai Acad Agr Sci, Inst Anim Husb & Vet Sci, Key Lab Livestock & Poultry Resources Pig Evaluat, Minist Agr & Rural Affairs, Shanghai 201106, Peoples R China
关键词： Porcine oocytes; Meiosis; Ran-GTP; Importin beta; Spindle
期刊名称：HISTOCHEMISTRY AND CELL BIOLOGY （ 影响因子：2.531； 五年影响因子：3.617 ）
ISSN： 0948-6143
年卷期：
页码：
收录情况： SCI
摘要： The Ran-GTP/importin beta pathway has been implicated in a diverse array of mitotic functions in somatic mitosis; however, the possible meiotic roles of Ran-GTP/importin beta in mammalian oocyte meiosis are still not fully understood. In the present study, importazole (IPZ), a small molecule inhibitor of the interaction between Ran and importin beta was used to explore the potential meiotic roles of Ran-GTP/importin beta in porcine oocytes undergoing meiosis. After IPZ treatment, the extrusion rate of the first polar body (PB1) was significantly decreased, and a higher proportion of the oocytes were arrested at the germinal vesicle breakdown (GVBD) stage. Moreover, IPZ treatment led to severe defects in metaphase I (MI) spindle assembly and chromosome alignment during the germinal vesicle (GV)-to-MI stage, as well as failure of metaphase II (MII) spindle reassembly and homologous chromosome segregation during the MI-to-MII stage. Notably, IPZ treatment decreased TPX2 expression and abnormal subcellular localization. Furthermore, the expression levels of aurora kinase A (AURKA) and transforming acidic coiled-coil 3 (TACC3) were significantly reduced after IPZ treatment. Collectively, these data indicate that the interaction of Ran-GTP and importin beta is essential for proper spindle assembly and successful chromosome segregation during two consecutive meiotic divisions in porcine oocytes, and regulation of this complex might be related to its effect on the TPX2 signaling cascades. [GRAPHICS] .
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