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Comparative proteomic analysis of wild-type and a SlETR-3 (Nr) mutant reveals an ethylene-induced physiological regulatory network in fresh-cut tomatoes

文献类型： 外文期刊

作者： Shi, Jun-yan 1 ; Wang, Qian 1 ; Zuo, Jin-hua 1 ; Zheng, Shu-fang 1 ; Gao, Li-pu 1 ; Liu, Jia 1 ; Wang, Qing 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Agrifood Proc & Nutr IAPN, Key Lab Vegetable Postharvest Treatment, Beijing Key Lab Fruits & Vegetable Storage & Proc,, Beijing 100097, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Vegetables, Key Lab Biol Genet Improvement Hort Crops North Ch, Key Lab Urban Agr North,Minist Agr, Beijing 100097, Peoples R China

3.Tianjin Acad Agr Sci, Biotechnol Res Inst, Tianjin 300381, Peoples R China

4.Chongqing Univ Arts & Sci, Inst Special Plants, Coll Landscape Architecture & Life Sci, Chongqing Key Lab Econ Plant Biotechnol, Chongqing 402160, Peoples R China

5.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Dept Food Sci & Engn, Beijing Key Lab Forest Food Proc & Safety, Beijing 100083, Peoples R China

关键词： Fresh-cut tomato; Proteomics; Ethylene; Pectinesterase; Physiological regulatory network

期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：7.425； 五年影响因子：7.716 ）

ISSN： 0963-9969

年卷期： 2022 年 161 卷

页码：

收录情况： SCI

摘要： Ethylene plays a crucial role in regulating fruit ripening, quality, and defense response. However, the mechanism (s) responsible for wound-induced ethylene regulation of fruit physiology at a network level is unclear. We used mass spectrometry (MS) to identify differences in the physiological response between fresh-cut fruits of wild-type (WT) tomato and an ethylene receptor mutant (SlETR-3) (also referred to as Nr) during storage. We found that Nr mutants exhibited better appearance and quality, as well as higher ethylene levels during the first 3 d of storage at 4 degrees C. Thirty-seven (0 h), eighty-two (12 h) and twelve (24 h) differentially abundant proteins were identified between the fresh-cut slices of the two genotypes during storage at the designated timepoints. In particular, antioxidant enzymes, such as ascorbate peroxidase, glutathione S-transferase, and peroxiredoxin were highly expressed in WT fruit, which was associated with higher H2O2 production, and high levels of transcription of cell -wall degrading enzymes. Leucine aminopeptidase, a marker enzyme for response to wounding exhibited higher levels in the Nr mutant, which is consistent with its higher production of ethylene. Collectively, our results provide a deeper insight into the ethylene-induced physiological regulatory network that is activated in fresh-cut tomatoes.

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