文献类型： 外文期刊
作者： Li, Fangying 1 ; Fu, Xiaozhe 2 ; Luo, Xia 2 ; Lin, Qiang 2 ; Liang, Hongru 2 ; Niu, Yinjie 2 ; Liu, Lihui 2 ; Li, Ningqiu 2 ;
作者机构： 1.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai, Peoples R China
2.Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Key Lab Fishery Drug Dev, Guangdong Prov Key Lab Aquat Anim Immune Technol,M, Guangzhou, Peoples R China
关键词： Siniperca chuatsi rhabdovirus; asparagine; aspartate metabolism; aspartate-malate shuttle; viral replication
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.2； 五年影响因子：6.2 ）
ISSN：
年卷期： 2023 年 14 卷
页码：
收录情况： SCI
摘要： Viruses are non-living organisms that rely on host cellular metabolism to complete their life cycle. Siniperca chuatsi rhabdovirus (SCRV) has caused huge economic losses to the Chinese perch (Siniperca chuatsi) industry worldwide. SCRV replication is dependent on the cellular glutamine metabolism, while aspartate metabolism plays an important role in viral proliferation in glutamine deficiency. Herein, we investigated roles of asparagine metabolism in SCRV proliferation. Results showed that SCRV infection upregulated the expression of key enzymes in the aspartate metabolic pathway in CPB cells. And the key enzymes of malate-aspartic acid shuttle pathway upregulated during the virus invasion phase, and key enzymes of the asparagine biosynthesis pathway upregulated during the viral replication and release phase. When asparagine was added to the depleted medium, the SCRV copy number restored to 90% of those in replete medium, showing that asparagine and glutamine completely rescue the replication of SCRV. Moreover, inhibition of the aspartate- malate shuttle pathway and knockdown of the expression of key enzymes in the asparagine biosynthesis pathway significantly reduced SCRV production, indicating that the aspartic acid metabolic pathway was required to the replication and proliferation of SCRV. Above results provided references for elucidating pathogenic mechanism of SCRV by regulation of aspartate metabolism.
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