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Functional characterization of a novel violacein biosynthesis operon from Janthinobacterium sp. B9-8

文献类型： 外文期刊

作者： Xu, Xian 1 ; Chu, Xiaoting 2 ; Du, Bangmian 1 ; Huang, Chunyan 1 ; Xie, Chengjia 3 ; Zhang, Zhidong 4 ; Jiang, Ling 5 ;

作者机构： 1.Nanjing Normal Univ, Sch Food Sci & Pharmaceut Engn, Nanjing 210046, Jiangsu, Peoples R China

2.Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, State Key Lab Mat Oriented Chem Engn, Nanjing 211816, Jiangsu, Peoples R China

3.Yangzhou Polytech Inst, Sch Chem Engn, Yangzhou 225127, Jiangsu, Peoples R China

4.Xinjiang Acad Agr Sci, Inst Microbiol, Urumqi 830091, Xinjiang Uyghur, Peoples R China

5.Nanjing Tech Univ, Coll Food Sci & Light Ind, Nanjing 211816, Jiangsu, Peoples R China

关键词： Violacein; High-throughput sequencing; Operon; Escherichia coli; Janthinobacterium

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：5.56； 五年影响因子：5.365 ）

ISSN： 0175-7598

年卷期： 2022 年 106 卷 8 期

页码：

收录情况： SCI

摘要： Violacein is a secondary metabolite mainly produced by Gram-negative bacteria that is formed from tryptophan by five enzymes encoded by a single operon. It is a broad-spectrum antibacterial pigment with various important biological activities such as anti-tumor, antiviral, and antioxidative effects. The newly discovered violacein operon vioABCDE was identified in the genome of the extremophile Janthinobacterium sp. B9-8. The key enzyme-encoding genes were cloned to construct the multigene coexpression plasmids pET-vioAB and pRSF-vioCDE. The violacein biosynthesis pathway was heterologously introduced into engineered Escherichia coli VioABCDE and VioABCDE-SD. The factors affecting violacein production, including temperature, pH, inoculum size, carbon and nitrogen source, precursor, and inducers were investigated. The violacein titer of VioABCDE-SD reached 107 mg/L in a two-stage fermentation process, representing a 454.4% increase over the original strain. The violacein operon from B9-8 provides a new microbial gene source for the analysis of the violacein synthesis mechanism, and the constructed engineering E. coli strains lay a foundation for the efficient and rapid synthesis of other natural products.

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