文献类型： 外文期刊
作者： Huang, Xuan-yun 1 ; Huang, Dong-mei 1 ; Cai, You-qiong 1 ;
作者机构： 1.Minist Agr PR China, CAFS Aquat Prod Qual Inspect & Test Ctr Shanghai, East China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China
关键词： Nitrofurazone;Semicarbazide;Hapten;Conjuguate;Polyclonal Antibody
期刊名称：PROCEEDINGS OF THE 3RD INTERNATIONAL CONFERENCE ON MATERIAL, MECHANICAL AND MANUFACTURING ENGINEERING
ISSN： 2352-5401
年卷期： 2015 年 27 卷
页码：
收录情况： SCI
摘要： Nitrofurazone residues in fishery products were dangerous for human health, semicarbazide (SEM) was the nitrofurazone metabolite could residues in fishery in a long time. To detect SEM residues in fishery products rapidly by immune method, preparation of high quality polyclonal antibody against SEM was the most critical step of project. We produced two kinds of antigens for SEM, using carrier protein bovine serum albumin (BSA) and ovalbumin (OVA) by a modified carbodiimide method, and then the hapten-protein conjugates were characterized by ultraviolet spectrophotometry in detecting qualitatively comparable hapten density, before being used for the immunization and detection purposes. The production of polyclonal antibodies (PcAbs) were sought following the generation of appropriate SEM-BSA conjugate. One PcAb against Semicarbazide (SEM) was produced and the titer polyclonal antibody could reach 10(5). In the optimized ELISA, the PcAb showed 50% inhibition at 25.0ng/mL for SEM in buffer.
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