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Pulsed Light Mutagenesis of the Willow Bracket Mushroom, Phellinus igniarius (Agaricomycetes), for Enhanced Production of Flavonoids, Laccase, and Fermentation Biomass

文献类型： 外文期刊

作者： Dong, Yating 1 ; Ma, Haile 1 ; Golly, Moses Kwaku 3 ; Qu, Wenjuan 1 ; Wang, Bei 1 ; Zhou, Cunshan 1 ; Ye, Xiaofei 4 ; Zhang, Henan 5 ; Gan, Bingcheng 2 ; Yan, Junjie 2 ; Han, Xing 2 ; Zhao, Jin 2 ; Feng, Rencai 2 ; Miao, Renyun 2 ; Zhang, Zhiyuan 7 ;

作者机构： 1.Jiangsu Univ, Inst Food Phys Proc, Int Joint Res Ctr Food Phys Proc, Sch Food & Biol Engn, 301 Xuefu Rd, Zhenjiang 212013, Peoples R China

2.Chinese Acad Agr Sci, Inst Urban Agr, Natl Agr Sci & Technol Ctr NASC, Tianfu New Area, 9 Hupan West Rd, Chengdu 610000, Peoples R China

3.Sunyani Tech Univ, Fac Appl Sci & Technol, Sunyani, Ghana

4.Univ Tennessee, Dept Biosyst Engn & Soil Sci, Knoxville, TN 37996 USA

5.Minist Agr, Natl Engn Res Ctr Edible Fungi, Key Lab Appl Mycol Resources & Utilizat, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201403, Peoples R China

6.Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai 201403, Peoples R China

7.Sichuan Acad Agr Sci, Inst Agr Resources & Environm, Inst Edible Fungi, Shizishan Rd 4, Chengdu 610066, Peoples R China

8.Jiangsu Univ, Sch Food & Biol Engn, 301 Xuefu Rd, Zhenjiang 212013, Peoples R China

关键词： Phellinus igniarius; pulsed light; flavonoids; laccase activity; antagonistic; RAPD; medicinal mushrooms

期刊名称：INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS （ 影响因子：1.706； 五年影响因子：1.817 ）

ISSN： 1521-9437

年卷期： 2022 年 24 卷 10 期

页码：

收录情况： SCI

摘要： Phellinus igniarius is a medicinal fungus possessing potent therapeutic activity due to the polysaccharides, polyphenols, flavonoids, and other secondary metabolites they contain. Laccases are crucial enzymes involved in lignin degradation in Ph. igniarius and offer great potential to accomplish several bioprocesses. To generate Ph. igniarius strains with high biomass, flavonoid, and laccase activity, we used pulsed light (PL) technology for mutagenesis of Ph. igniarius protoplasts and screened for mutants with high biomass, flavonoid, and laccase activity. At the irradiation power of 100 J, treated distance 8.5 cm, irradiation frequency was 0.5 s/time, three times treatments, after five generations of selection, three mutants were obtained with higher biomass production. Compared with control, the mycelium biomass and the flavonoid production of the screened mutant strain QB72 were increased 20.87% and 53.51%, respectively. The total amount of the accumulated extracellular laccase of the QB72 in the first 6 and 8 days increased 23.38% and 22.37% respectively, and over the total 16 days it increased 9.62%. In addition, RAPD analysis results indicated that the genetic materials of the mutant QB72 were altered. PL mutagenesis method has great potential for developing strains, especially Phellinus.

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