DEVELOPMENT OF FUNCTIONAL MARKERS FOR QUANTITATIVE TRAIT LOCUS UNDERLYING RICE QUALITY COMPONENTS
文献类型： 外文期刊
作者： Mao, Ting 1 ; Li, Xu 1 ; Xu, Zhengjin 2 ; Wang, Jiayu 2 ; Tang, Liang 2 ; Xu, Hai 1 ; Jiang, Shukun 3 ; Zhang, Zhan 1 ; Li, Zhenyu 1 ;
作者机构： 1.Liaoning Prov Saline & Alkaline Land Utilizat & R, Panjin 124010, Peoples R China
2.Shenyang Agr Univ, Rice Res Inst, Minist Educ, Key Lab Northern Japon Rice Genet & Breeding, Shenyang 110866, Liaoning, Peoples R China
3.Heilongjiang Acad Agr Sci, Cultivat & Farming Res Inst, Harbin 150086, Heilongjiang, Peoples R China
关键词： Rice quality; GS3; Chalk5; Wx; Functional markers; Tetra-primer ARMS-PCR
期刊名称：PAKISTAN JOURNAL OF BOTANY （ 影响因子：0.972； 五年影响因子：0.988 ）
ISSN： 0556-3321
年卷期： 2018 年 50 卷 2 期
页码：
收录情况： SCI
摘要： Three locus (GS3, Chalk5 and Wx) controlling grain size, grain chalkiness and amylose content (AC) have been cloned and can be used to improve rice quality by molecular marker-assisted selection (MAS). At present, identification of the above-mentioned alleles need DNA sequencing or restriction enzyme digestion, which were laborious, time-consuming tasks. Developing functional markers harboring the advantage of rapid, simple and low cost will contribute to rice quality improvement by MAS. Tetra-primer ARMS-PCR technology is an economical method for single nucleotide polymorphisms (SNP) scoring, however, its utilization is limited due to low amplification efficiency and inaccuracy extension caused by distinct melting temperature (Tm) difference existing between four primers in one PCR system. In this paper, we developed a set of functional markers using tetra-primer ARMS-PCR technology, and we also put forward corresponding solving schemes on low amplification efficiency and inaccuracy extension caused by Tm difference. The results showed that five markers were developed through adjusting the location of deliberate mismatch bases introduced, three markers (GS3(ac )-ARMS, Chalk5(tc)-ARMS, Waxy(gt)-ARMS) could identify different alleles for each loci holding the advantage of rapid, simple and low cost. Further, we analyzed the alleles distribution on rice varieties derived from Northeast China. All of these works could provide foundations for rice quality improvement utilizing the suitable genes (QTLs) by MAS.
- 相关文献
作者其他论文 更多>>
-
Chilling at grain filling stage reduced rice grain protein content: An experimental and modeling study
作者：Ma, Haoyu;Shi, Yanying;Guo, Erjing;Guan, Kaixin;Zhou, Litao;Deng, Yuhan;Liu, Runze;Zhang, Wenmeng;Zhao, Yuanbo;Zheng, Xinyi;Li, Mingzhe;Yang, Chenlong;Yang, Xiaoguang;Li, Tao;Wang, Lizhi;Zhang, Xijuan;Yang, Xianli;Jiang, Shukun;Cui, Shize
关键词：Rice; Chilling stress; Grain filling stage; Grain protein; Nitrogen accumulation; Crop model
-
Response of rice yield, grain quality to chilling at grain filling stages
作者：Guo, Erjing;Shi, Yanying;Cheng, Xue;Chen, Xi;Ma, Haoyu;Wan, Nenhan;Bai, Fan;Zhang, Zhentao;Yang, Xiaoguang;Wang, Lizhi;Jiang, Shukun;Zhang, Tianyi;Li, Tao
关键词：rice; chilling; grain filling stage; yield; grain quality
-
A Single Nucleotide Variation of CRS2 Affected the Establishment of Photosynthetic System in Rice
作者：Chen, Hongwei;Wang, Qi;Fan, Mingqian;Feng, Pulin;Zhu, Lin;Wu, Jiayi;Cheng, Xiaoyi;Wang, Jiayu;Zhang, Xijuan
关键词：chloroplast RNA splicing; photosynthesis; chlorophyll fluorescence; map-based cloning; rice (Oryza sativa L.)
-
An experimental study on the effects of intermittent chilling at different growth stages on rice yield in Northeast China
作者：Shi, Yanying;Guo, Erjing;Zhou, Litao;Zhang, Wenmeng;Ma, Haoyu;Guan, Kaixin;Li, E.;Yang, Xiaoguang;Wang, Lizhi;Jiang, Shukun;Li, Tao;Zhang, Tianyi
关键词：grain yield; growth stages; intermittent chilling; photosynthesis; rice
-
Isolation of a Novel QTL, qSCM4, Associated with Strong Culm Affects Lodging Resistance and Panicle Branch Number in Rice
作者：Yang, Xianli;Lai, Yongcai;Wang, Lizhi;Li, Mingxian;Chi, Liyong;Lv, Guoyi;Liu, Youhong;Li, Rui;Wu, Liren;Sun, Bing;Zhang, Xijuan;Yang, Xianli;Lai, Yongcai;Wang, Lizhi;Li, Mingxian;Chi, Liyong;Li, Rui;Zhang, Xijuan;Jiang, Shukun;Yang, Xianli;Lai, Yongcai;Wang, Lizhi;Liu, Youhong;Zhang, Xijuan;Jiang, Shukun;Zhao, Minghui;Wang, Jiayu;Jiang, Shukun
关键词：rice; lodging resistance; functional analysis; qSCM4; candidate gene
-
A chromosome-level genome assembly of an early matured aromatic Japonica rice variety Qigeng10 to accelerate rice breeding for high grain quality in Northeast China
作者：Jiang, Shukun;Liu, Chuanzeng;Ma, Bo;Miao, Yi;Hu, Jifang;Tan, Kefei;Wang, Yuxian;Jiang, Shukun;Zhang, Xijuan;Yang, Xianli;Wang, Lizhi;Wang, Junhe;Jiang, Shukun;Zhang, Xijuan;Yang, Xianli;Liu, Chuanzeng;Wang, Lizhi;Ma, Bo;Miao, Yi;Hu, Jifang;Tan, Kefei;Wang, Yuxian;Zhang, Xijuan;Yang, Xianli;Wang, Lizhi;Wang, Junhe;Jiang, Hui
关键词：aromatic japonica rice; genome assembly; early-matured; northern limit region; functional genes
-
Impacts of chilling at the tillering phases on rice growth and grain yield in Northeast China
作者：Guo, Erjing;Shi, Yanying;Chen, Xi;Cheng, Xue;Wang, Xiaoyu;Yang, Xiaoguang;Wang, Lizhi;Jiang, Shukun;Xiang, Hongtao;Wang, Lianmin;Feng, Yanjiang;Lai, Yongcai;Chen, Xi;Cheng, Xue;Wang, Xiaoyu;Zhang, Tianyi;Li, Tao
关键词：chilling; grain yield; rice; tillering phase; yield components
