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Expression and Immunogenicity of SARS-CoV-2 Virus-Like Particles based on Recombinant Truncated HEV-3 ORF2 Capsid Protein

文献类型： 外文期刊

作者： Zhou, Yong-Fei 1 ; Nie, Jiao-Jiao 1 ; Shi, Chao 1 ; Ning, Ke 1 ; Cao, Yu-Feng 4 ; Xie, Yanbo 5 ; Xiang, Hongyu 1 ; Xie, Qiuhong 1 ;

作者机构： 1.Jilin Univ, Sch Life Sci, Natl Engn Lab AIDS Vaccine, Changchun 130012, Jilin, Peoples R China

2.Jilin Univ, Sch Life Sci, Changchun 130012, Peoples R China

3.Jilin Univ, Inst Changbai Mt Resource & Hlth, Jilin 134504, Peoples R China

4.Immune Path Biotechnol Suzhou Co Ltd, Suzhou 215000, Peoples R China

5.Jilin Acad Agr Sci, Jilin Prov Key Lab Agr Biotechnol, Changchun 130033, Peoples R China

关键词： SARS-CoV-2; recombinant vaccine; virus-like nanoparticles; hepatitis E virus

期刊名称：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：3.277； 五年影响因子：3.08 ）

ISSN： 1017-7825

年卷期： 2022 年 32 卷 10 期

页码：

收录情况： SCI

摘要： COVID-19 is an emerging disease that poses a severe threat to global public health. As such, there is an urgent demand for vaccines against SARS-CoV-2, the virus that causes COVID-19. Here, we describe a virus-like nanoparticle candidate vaccine against SARS-CoV-2 produced by an E. coli expression system. The fusion protein of a truncated ORF2-encoded protein of aa 439 similar to 608 (p170) from hepatitis E virus CCJD-517 and the receptor-binding domain of the spike protein from SARS-CoV-2 were expressed, purified and characterized. The antigenicity and immunogenicity of p170-RBD were evaluated in vitro and in Kunming mice. Our investigation revealed that p170-RBD self-assembled into approximately 24 nm virus-like particles, which could bind to serum from vaccinated people (p < 0.001) and receptors on cells. Immunization with p170-RBD induced the titer of IgG antibody vaccine increased from 14 days post-immunization and was significantly enhanced after a booster immunization at 28 dpi, ultimately reaching a peak level on 42 dpi with a titer of 4.97 log(10). Pseudovirus neutralization tests showed that the candidate vaccine induced a strong neutralizing antibody response in mice. In this research, we demonstrated that p170-RBD possesses strong antigenicity and immunogenicity and could be a potential candidate for use in future SARS-CoV-2 vaccine development.

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