Cyprinid herpesvirus 2 infection emerged in cultured gibel carp, Carassius auratus gibelio in China
文献类型: 外文期刊
作者: Xu, Jin 1 ; Zeng, Lingbing 1 ; Zhang, Hui 1 ; Zhou, Yong 1 ; Ma, Jie 1 ; Fan, Yuding 1 ;
作者机构: 1.Chinese Acad Fishery Sci, Minist Agr China, Key Lab Yangtze River Basins Aquat Anim Dis, Div Fish Dis Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China
关键词: Gibel carp (Carassius auratus gibelio);Epizootic;Cyprinid herpesvirus 2;Pathogenicity;Isolation;Identification
期刊名称:VETERINARY MICROBIOLOGY ( 影响因子:3.293; 五年影响因子:3.599 )
ISSN: 0378-1135
年卷期: 2013 年 166 卷 1-2 期
页码:
收录情况: SCI
摘要: An epizootic with severe mortality has emerged in cultured gibel carp, Carassius auratus gibelio, in China since 2009, and caused huge economic loss. The signs and epidemiology background of the disease were investigated. Parasite examination, bacteria and virus isolation were carried out for pathogen isolation. The causative pathogen was obtained and identified as Cyprinid herpesvirus 2 (CyHV-2) by experimental infection, electron microscopy, cell culture, PCR assay and sequence alignment, designated as CyHV-2-JSSY. Experimental infection proved the high virulence of CyHV-2-JSSY to healthy gibel carp. Electron microscopy revealed that the viral nucleocapsid was hexagonal in shape measuring 110-120 nm in diameter with a 170-200 nm envelope. The virus caused significant CPE in Koi-Fin cells at the early passages, but not beyond the fifth passages. Sequence alignment of the partial viral helicase gene (JX566884) showed that it shared 99-100% identity to the published sequences of other CyHV-2 isolates. This study represented the first isolation and identification of CyHV-2 in cultured gibel carp in China and laid a foundation for the further studies of the disease. (C) 2013 Elsevier B.V. All rights reserved.
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