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miR-8-3p regulates the antioxidant response and apoptosis in white shrimp, vannamei under ammonia-N stress

文献类型： 外文期刊

作者： Zhuo, Hongbiao 1 ; Zhang, Yuan 1 ; Fu, Shuo 1 ; Lin, Lanting 1 ; Li, Jinyan 1 ; Zhou, Xiaoxun 1 ; Wu, Guangbo 1 ; Guo, Chaoan 1 ; Liu, Jianyong 1 ;

作者机构： 1.Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Qingdao 266071, Shandong, Peoples R China

关键词： Litopenaeus vannamei; Lva-miR-8-3p; Ammonia -N stress; Antioxidant response; Apoptosis

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：7.7； 五年影响因子：7.7 ）

ISSN： 0141-8130

年卷期： 2024 年 274 卷

页码：

收录情况： SCI

摘要： Exposure to excess ammonia-N (NH3/NH4+) in aquaculture can disrupt physiological function in shrimp leading to enhanced oxidative stress and apoptosis, but little is known concerning the post-transcriptional regulation mechanism. In this study, the first miR-200 family member in crustacean was identified and characterized from Litopenaeus vannamei (designed as Lva-miR-8-3p). Lva-miR-8-3p was highly expressed in eyestalks, brainganglion, and gills. The expression of Lva-miR-8-3p in gills significantly decreased after ammonia-N stress, and LvamiR-8-3p was confirmed to target IKK beta 3'UTR for negatively regulating IKK beta/NF-kappa B pathway. Overexpression of miR-8-3p promoted the hemolymph ammonia-N accumulation, total hemocyte count (THC) decrease, and gills tissue damage, thus resulting in a decreased survival rate of ammonia-exposed shrimp. Besides, Lva-miR-8-3p silencing could enhance the antioxidant enzymes activities and reduce the oxidative damage, whereas overexpression of Lva-miR-8-3p exerted the opposite effects. Furthermore, Lva-miR-8-3p overexpression was found to aggravate ammonia-N induced apoptosis in gills. In primarily cultured hemocytes, the cell viability decreased, the ROS content and caspase-3 activity increased after agomiR-8-3p transfection, while antagomiR-8-3p transfection caused the opposite change except the cell viability. These findings indicate that Lva-miR-8-3p acts as a post-transcriptional regulator in ammonia-N induced antioxidant response and apoptosis by negatively regulating IKK beta/NF-kappa B pathway.

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