您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Efficacy of recombinant Newcastle disease virus expressing HA protein of H9N2 Avian influenza virus in respiratory and intestinal tract

文献类型： 外文期刊

作者： Shao, Guanming 1 ; Xie, Zi 1 ; Liang, Ming 1 ; Liu, Yaxin 1 ; Song, Chaoyi 6 ; Feng, Keyu 1 ; Zhang, Xinheng 1 ; Lin, Wencheng 1 ; Fu, Jun 6 ; Xie, Qingmei 1 ;

作者机构： 1.South China Agr Univ, Heyuan Branch, Guangdong Prov Lab Lingnan Modern Agr Sci & Techn, Coll Anim Sci, Guangzhou 510642, Peoples R China

2.South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Coll Anim Sci, Guangzhou 510642, Peoples R China

3.Guangdong Engn Res Ctr Vector Vaccine Anim Virus, Guangzhou 510642, Peoples R China

4.South China Collaborat Innovat Ctr Poultry Dis Co, Guangzhou 510642, Peoples R China

5.Key Lab Anim Hlth Aquaculture & Environm Control, Guangzhou 510642, Guangdong, Peoples R China

6.Shandong Univ, Helmholtz Inst Biotechnol, State Key Lab Microbial Technol, Helmholtz Int Lab Antiinfect,Inst Microbial Techn, Qingdao, Peoples R China

关键词： H9N2 avian influenza virus; Newcastle disease virus; HA protein; trachea; intestine

期刊名称：POULTRY SCIENCE （ 影响因子：4.4； 五年影响因子：4.4 ）

ISSN： 0032-5791

年卷期： 2022 年 101 卷 12 期

页码：

收录情况： SCI

摘要： H9N2 subtype avian influenza virus (AIV) is a low pathogenic AIV, which is widely prevalent all over the world. The infection of H9N2 AIV often leads to secondary infection with other pathogens, causing serious economic losses to poultry industry. Up to now, several recombinant Newcastle disease viruses (NDV) expressing H9N2 AIV hemagglutinin (HA) protein had been developed. However, the efficacy of recombinant virus on tracheal and intestinal injury caused by H9N2 AIV was rarely reported. The aim of this study was to evaluate the efficacy of recombinant NDV expressing H9N2 AIV HA protein in respiratory and intestinal tract. In this study, based on Red/ET homologous recombination technology, H9N2 AIV HA gene was embedded into the genome of NDV LaSota vaccine strain to obtain the recombinant virus rNDV-H9. The recombinant virus rNDV-H9 showed similar replication kinetic characteristics with the parent LaSota strain and had good genetic stability. The immunization result showed that rNDV-H9 induced high HI antibody titer against H9N2 AIV. In the H9N2 AIV challenge experiment, rNDV-H9 could significantly reduce the virus shedding in trachea and cloaca. In addition, rNDV-H9 protected the barrier function of chicken intestinal mucosal epithelial cells and reduced the virus-induced inflammatory response to a certain extent, so as to inhibit the abnormal proliferation of E. coli. This study suggests that rNDV-H9 is a promising vaccine candidate against H9N2 AIV.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台