Quantitative proteomic analysis and verification identify global protein profiling dynamics in pig during the estrous cycle
文献类型： 外文期刊
作者： Xin, Haiyun 1 ; Li, Baohong 1 ; Meng, Fanming 1 ; Hu, Bin 1 ; Wang, Sutian 1 ; Wang, Ying 3 ; Li, Jianhao 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Sci, State Key Lab Swine & Poultry Breeding Ind, Guangdong Key Lab Anim Breeding & Nutr, Guangzhou, Peoples R China
2.Guangdong Lab Lingnan Modern Agr, Maoming Branch, Maoming, Peoples R China
3.Guangzhou Customs Tech Ctr, Guangzhou, Peoples R China
关键词： sow; estrus detection; saliva; quantitative proteomics; differentially expressed proteins; reproduction efficiency
期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：3.2； 五年影响因子：3.5 ）
ISSN：
年卷期： 2023 年 10 卷
页码：
收录情况： SCI
摘要： The current estrus detection method is generally time-consuming and has low accuracy. As such, a deeper understanding of the physiological processes during the estrous cycle accelerates the development of estrus detection efficiency and accuracy. In this study, the label-free acquisition mass spectrometry was used to explore salivary proteome profiles during the estrous cycle (day -3, day 0, day 3, and day 8) in pigs, and the parallel reaction monitoring (PRM) was applied to verify the relative profiles of protein expression. A total of 1,155 proteins were identified in the label-free analysis, of which 115 were identified as differentially expressed proteins (DEPs) among different groups (p <= 0.05). Functional annotation revealed that the DEPs were clustered in calcium ion binding, actin cytoskeleton, and lyase activity. PRM verified the relative profiles of protein expression, in which PHB domain-containing protein, growth factor receptor-bound protein 2, elongation factor Tu, carboxypeptidase D, carbonic anhydrase, and trefoil factor 3 were confirmed to be consistent in both label-free and PRM approaches. Comparative proteomic assays on saliva would increase our knowledge of the estrous cycle in sows and provide potential methods for estrus detection.
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