您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Evaluation of humoral and cellular immune responses induced by a cocktail of recombinant African swine fever virus antigens fused with OprI in domestic pigs

文献类型： 外文期刊

作者： Zhang, Guanglei 1 ; Liu, Wei 1 ; Yang, Sicheng 1 ; Song, Shuai 2 ; Ma, Yunyun 1 ; Zhou, Guangqing 1 ; Liang, Xiaxia 1 ; Miao, Chun 1 ; Li, Junhui 1 ; Liu, Yanhong 1 ; Shao, Junjun 1 ; Chang, Huiyun 1 ;

作者机构： 1.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Anim Dis Control & Prevent, OIE China Natl Foot & Mouth Dis Reference Lab, Lanzhou, Gansu, Peoples R China

2.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou, Peoples R China

3.China Natl Biotec Grp Co Ltd CNBG, Lanzhou Inst Biol Prod Co Ltd LIBP, Lanzhou 730046, Peoples R China

关键词： African swine fever virus; Recombinant fusion proteins; OprI; Immune responses; Vaccine

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.8； 五年影响因子：3.9 ）

ISSN：

年卷期： 2023 年 20 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundAfrican swine fever (ASF) is a highly fatal disease in domestic pigs caused by ASF virus (ASFV), for which there is currently no commercial vaccine available. The genome of ASFV encodes more than 150 proteins, some of which have been included in subunit vaccines but only induce limited protection against ASFV challenge.MethodsTo enhance immune responses induced by ASFV proteins, we expressed and purified three fusion proteins with each consisting of bacterial lipoprotein OprI, 2 different ASFV proteins/epitopes and a universal CD4(+) T cell epitope, namely OprI-p30-modified p54-TT, OprI-p72 epitopes-truncated pE248R-TT, and OprI-truncated CD2v-truncated pEP153R-TT. The immunostimulatory activity of these recombinant proteins was first assessed on dendritic cells. Then, humoral and cellular immunity induced by these three OprI-fused proteins cocktail formulated with ISA206 adjuvant (O-Ags-T formulation) were assessed in pigs.ResultsThe OprI-fused proteins activated dendritic cells with elevated secretion of proinflammatory cytokines. Furthermore, the O-Ags-T formulation elicited a high level of antigen-specific IgG responses and interferon-gamma-secreting CD4(+) and CD8(+) T cells after stimulation in vitro. Importantly, the sera and peripheral blood mononuclear cells from pigs vaccinated with the O-Ags-T formulation respectively reduced ASFV infection in vitro by 82.8% and 92.6%.ConclusionsOur results suggest that the OprI-fused proteins cocktail formulated with ISA206 adjuvant induces robust ASFV-specific humoral and cellular immune responses in pigs. Our study provides valuable information for the further development of subunit vaccines against ASF.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台