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The effect of hyperthermia on cell viability, oxidative damage, and heat shock protein expression in hepatic cells of grass carp (Ctenopharyngodon idellus)

文献类型： 外文期刊

作者： Cui, Yanting 1 ; Liu, Bo 1 ; Xie, Jun 1 ; Xu, Pao 1 ; Tsion, H. Michael H. 1 ; Zhang, Yuanyuan 1 ;

作者机构： 1.Nanjing Agr Univ, Wuxi Fishery Coll, Wuxi 214081, Peoples R China

2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Minist Agr, Key Lab Freshwater Fisheries & Germplasm Resource, Wuxi 214081, Peoples R China

3.Chinese Acad Fishery Sci,

关键词： Hyperthermia;Oxidative stress;HSPs;Hepatic cell;Grass carp

期刊名称：JOURNAL OF THERMAL BIOLOGY （ 影响因子：2.902； 五年影响因子：3.102 ）

ISSN： 0306-4565

年卷期： 2013 年 38 卷 6 期

页码：

收录情况： SCI

摘要： The purpose of the study was to investigate the effects of mild hyperthermia on cell viability, release of lactate dehydrogenase (LDH), superoxide dismutase (SOD) activity, malondialdehyde (MDA) formation, total antioxidant capacity (T-AOC), and the relative mRNA levels of heat shock protein (HSP60, 70, and 90) in hepatic cells of grass carp (Ctenopharyngodon idellus) before and after temperature stress. Cultured cells were exposed to thermal stress (32 degrees C) for 0.5, 1, 2, 4, and 8 h. The results showed that hyperthermia stress significantly reduced cell viability (P < 0.01) and increased LDH release at 0.5 and 1 h (P < 0.05). Additionally, hyperthermia stress led to oxidative stress as evidenced by significantly decreased T-AOC after treating cells for 0.5 and 8 h (P < 0.05). SOD activity also significantly decreased after 1 h of stress (P < 0.05), but MDA formation increased after 8 h of stress (P < 0.05). This may be partly responsible for the lower cell viability and higher LDH release we observed. The differences between SOD activity, MDA formation, and T-AOC between the 2 h treatment group and the control were smaller than that of other groups. This indicated that cellular antioxidant enzyme systems play an important role in the defense against oxidative stress. Further tests showed that the expression of HSP60 at 1, 2, and 4 h (P < 0.05), HSP70 at 0.5 and 1 h (P < 0.01). and HSP90 at all time points after stress were higher (P < 0.01) than prestress levels. This suggested that HSPs possess the ability to modulate cellular anti-stress responses and play key roles in protecting organisms from heat stress. In conclusion, hyperthermia inhibits cell proliferation, induces cell oxidative stress, and enhances HSP expression in hepatic cells of grass carp. (C) 2013 Published by Elsevier Ltd.

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