Casein Kinase 2 Mediates Degradation of Transcription Factor Pcf1 during Appressorium Formation in the Rice Blast Fungus
文献类型： 外文期刊
作者： Huang, Pengyun 1 ; Li, Yan 1 ; Wang, Jing 1 ; Wang, Qing 1 ; Huang, Zhicheng 1 ; Liu, Xiaohong 2 ; Lin, Fucheng 2 ; Lu, Jianping 1 ;
作者机构： 1.Zhejiang Univ, Coll Life Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310058, Peoples R China
2.Zhejiang Univ, Inst Biotechnol, Hangzhou 310058, Peoples R China
3.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China
关键词： Magnaporthe oryzae; casein kinase 2; transcription factor; protein degradation; appressorium; rice blast
期刊名称：JOURNAL OF FUNGI （ 影响因子：5.724； 五年影响因子：6.413 ）
ISSN：
年卷期： 2022 年 8 卷 2 期
页码：
收录情况： SCI
摘要： The appressorium is a specialized structure that is differentiated from Magnaporthe oryzae spores that can infect host cells. In the process of cellular transformation from spore to appressorium, the contents inside the spores are transferred into appressoria, accompanied by major differences in the gene expression model. In this study, we reported a transcription factor (TF), Pcf1, which was depressed at the transcription level and degraded at the protein level in nuclei of incipient appressoria at four hpi (hours post inoculation). To investigate its degradation mechanism, the interacting proteins of Pcf1 were identified using an immunoprecipitation-mass spectrometry (IP-MS) assay. Yeast two-hybrid (Y2H) and co-IP (co-immunoprecipitation) assays confirmed that Pcf1 interacted with the casein kinase 2 (CK2) holoenzyme through direct combination with the CKb2 subunit. Moreover, Pcf1 was ubiquitinated in the hyphae. These changes in Pcf1 protein levels in nuclei provide a new clue of how TFs are degraded during appressorium formation: temporarily unnecessary TFs in spores are phosphorylated through interacting with CK2 enzyme and are then ubiquitinated and digested by the ubiquitin proteasome system (UPS).
- 相关文献
作者其他论文 更多>>
-
Hue-change coupled with CRISPR-Cas12a lateral flow assay for the semiquantitative detection of Salmo salar adulteration
作者：Yuan, Jingrui;Wang, Liu;He, Fang;He, Kaiyu;Wang, Hongmei;Xu, Xiahong;Yuan, Jingrui;Huang, Liang;Wang, Jing;Xu, Xiahong
关键词：Hue change; CRISPR-Cas12a; Lateral flow assay; Quantitative analysis; Salmo salar adulteration
-
The endosomal-vacuolar transport system acts as a docking platform for the Pmk1 MAP kinase signaling pathway in Magnaporthe oryzae
作者：Wang, Qing;Huang, Zhicheng;Li, Yan;Li, Hui;Lin, Fu-Cheng;Lu, Jianping;Wang, Jing;Cai, Yingying;Wang, Jiaoyu;Lin, Fu-Cheng;Huang, Pengyun;Liu, Xiaohong;Lin, Fu-Cheng
关键词：appressorium; endosome; MAPK; Pmk1; rice blast fungus; signal; ubiquitination; vacuole
-
A multilayered regulatory network mediated by protein phosphatase 4 controls carbon catabolite repression and de-repression in Magnaporthe oryzae
作者：Huang, Zhicheng;Wang, Qing;Li, Yan;Li, Hui;Lin, Fu-Cheng;Lu, Jianping;Huang, Pengyun;Liao, Jian;Liu, Xiaohong;Lin, Fu-Cheng;Wang, Jing;Cai, Yingying;Wang, Jiaoyu;Lin, Fu-Cheng
关键词：
-
Carbon Dots@Upconversion Nanoparticles: Conjugate Manipulation and Luminophor Confinement Enabling Aqueous-Phase Orthogonal Multicolor Phosphorescence-Upconversion Luminescence
作者：Shao, Kang;Jin, Yuqian;Pan, Zaifa;Shi, Yike;Chen, Jiahong;Wang, Jing;She, Yuanbin;Ye, Shiyi
关键词：aqueous-phase phosphorescence; carbon dots; multicolor luminescence; orthogonal luminescence; upconversion nanoparticles
-
Integrating RNA Interference and Nanotechnology: A Transformative Approach in Plant Protection
作者：Islam, Mohammad Shafiqul;Noman, Muhammad;Zhang, Zhen;Wang, Jing;Lu, Ziqi;Cai, Yingying;Wang, Yanli;Wang, Jiaoyu;Islam, Mohammad Shafiqul;Li, Bin;Islam, Mohammad Shafiqul;Li, Bin;Ahmed, Md Robel;Ahmed, Temoor;Ahmed, Temoor;Sarwar, Abul Khayer Mohammad Golam
关键词：fungal disease; nanotechnology; plant protection; plant-pathogen interactions; RNAi
-
Effects of fructooligosaccharides and Lactobacillus reuteri on the composition and metabolism of gut microbiota in students
作者：Xu, Xu;Qu, Daofeng;Xu, Xu;Fu, Hao;Quan, Huihui;Li, Yan;Chen, Qiaoyun;Pi, Xionge
关键词：
-
Comparative functional analysis of a new CDR1-like ABC transporter gene in multidrug resistance and virulence between Magnaporthe oryzae and Trichophyton mentagrophytes
作者：Wang, Jing;Xiao, Chenwen;Liang, Shuang;Noman, Muhammad;Cai, Yingying;Zhang, Zhen;Zhu, Xueming;Chai, Rongyao;Qiu, Haiping;Hao, Zhongna;Wang, Yanli;Wang, Jiaoyu;Sun, Guochang;Lin, Fucheng;Bao, Guolian
关键词：
MoCDR1 ;TmCDR1 ; ABC transporters;Magnaporthe oryzae ;Trichophyton mentagrophytes
