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Anti-inflammatory and antioxidant properties of rice bran oil extract in copper sulfate-induced inflammation in zebrafish (Danio rerio)

文献类型： 外文期刊

作者： Liu, Naicheng 1 ; Zhang, Peng 1 ; Xue, Mingyang 1 ; Zhang, Mengjie 1 ; Xiao, Zidong 1 ; Xu, Chen 1 ; Fan, Yuding 1 ; Liu, Wei 1 ; Wu, Yeying 4 ; Wu, Minglin 5 ; Zhang, Qinghua 2 ; Zhou, Yong 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China

2.Shanghai Ocean Univ, Key Lab Explorat & Utilizat Aquat Genet Resources, Minist Educ, Shanghai 201306, Peoples R China

3.Shanghai Ocean Univ, Natl Pathogen Collect Ctr Aquat Anim, Shanghai, Peoples R China

4.Wuhan Dynam Life Sci Co Ltd, Wuhan 430223, Peoples R China

5.Anhui Acad Agr Sci, Fisheries Res Inst, Hefei 230031, Peoples R China

关键词： Anti-inflammatory; Antioxidation; CuSO4; Rice bran oil; Tocotrienols; Zebrafish

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 1050-4648

年卷期： 2023 年 136 卷

页码：

收录情况： SCI

摘要： Tocotrienols have strong antioxidant properties; however, tocotrienol has not been investigated in detail in aquatic products. In this study, the anti-inflammatory and antioxidant activities of the tocotrienol-rich fraction from rice bran oil and its potential mechanism were verified in a zebrafish CuSO4 inflammation model. The in vitro antioxidant activity was evaluated using the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) stable radical method. The copper chelating activity was determined using the pyrocatechol violet method. Intracellular reactive ox-ygen species in zebrafish were detected using a fluorescent ROS probe. Transgenic Tg (lyz: DsRed2) zebrafish were used for neutrophil transmigration assays. The mRNA expression levels of antioxidant and pro -inflammatory factor genes were measured using quantitative real-time reverse transcription PCR. In the con-centration range tested, 100 mu g/mL TRF had the highest copper chelating activity (10%). TRF showed DPPH-free radical scavenging ability, which was 53% at 100 mu g/mL TRF. TRF effectively repressed ROS generation and inhibited neutrophil migration to the inflamed site. Moreover, TRF upregulated the expression of antioxidant genes sod and gpx4b, inhibited the expression of pro-inflammatory factors tnfa and il8, and suppressed CuSO4- induced inflammation. In conclusion, TRF has significant anti-inflammatory and antioxidant properties, which supports the use of TRF as an aquatic feed additive to improve the anti-inflammatory and antioxidant capacity of aquatic products.

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