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Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot Scophthalmus maximus responded to hypo-saline seawater

文献类型： 外文期刊

作者： Cui, Wenxiao 1 ; Ma, Aijun 2 ; Huang, Zhihui 2 ; Wang, Xin'an 2 ; Sun, Zhibin 2 ; Liu, Zhifeng 2 ; Zhang, Wei 2 ; Yang, J 1 ;

作者机构： 1.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Minist Educ, Shanghai 201306, Peoples R China

2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China

3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

4.Shandong Key Lab Marine Fisheries Biotechnol & Ge, Qingdao 266071, Peoples R China

5.Qingdao Key Lab Marine Fish Breeding & Biotechnol, Qingdao 266071, Peoples R China

6.Yantai Tianyuan Aquat Ltd Corp, Yantai 264003, Peoples R China

关键词： osmoregulation mechanisms; Scophthalmus maximus; hypo-saline; signal transduction; cell volume regulation; gene expression

期刊名称：JOURNAL OF OCEANOLOGY AND LIMNOLOGY （ 影响因子：1.265； 五年影响因子：1.276 ）

ISSN： 2096-5508

年卷期： 2020 年 38 卷 2 期

页码：

收录情况： SCI

摘要： Turbot harbor a relatively remarkable ability to adapt to opposing osmotic challenges and are an excellent model species to study the physiological adaptations of flounder associated with osmoregulatory plasticity. The kidney transcriptome of turbot treated 24 h in water of hypo-salinity(salinity 5) and seawater(salinity 30) was sequenced and characterized. In silico analysis indicated that all unigenes had significant hits in seven databases. The functional annotation analysis of the transcriptome showed that the immune system and biological processes associated with digestion, absorption, and metabolism played an important role in the osmoregulation of turbot in response to hypo-salinity. Analysis of biological processes associated with inorganic channels and transporters indicated that mineral absorption and bile secretion contributed to iono-osmoregulation resulting in cell volume regulation and cell phenotypic plasticity. Moreover, we analyzed and predicted the mechanisms of canonical signaling transduction. Biological processes involved in renin secretion, ECM-receptor interaction, adherens junction, and focal adhesion played an important role in the plasticity phenotype in hypo-stress, while the signal transduction network composed of the MAPK signaling pathway and PI3K-Akt signaling pathway with GABAergic synapse, worked in hypo-osmoregulation signal transduction in the turbot. In addition, analysis of the tissue specificity of targeted gene expression using qPCR during salinity stress was carried out. The results showed that the kidney, gill, and spleen were vital regulating organs of osmotic pressure, and the osmoregulation pattern of euryhaline fish differed among species.

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