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Changes in water loss and cell wall metabolism during postharvest withering of tobacco (Nicotiana tabacum L.) leaves using tandem mass tag-based quantitative proteomics approach

文献类型： 外文期刊

作者： Wu, Shengjiang 1 ; Cao, Gaoyi 3 ; Adil, Muhammad Faheem 6 ; Tu, Yonggao 2 ; Wang, Wei 1 ; Cai, Bin 5 ; Zhao, Degang 1 ; S 1 ;

作者机构： 1.Guizhou Univ, Ctr Res & Dev Fine Chem, State Key Lab Breeding Base Green Pesticide & Agr, Minist Educ,Key Lab Plant Resources Conservat & G, Guiyang 550025, Peoples R China

2.Guizhou Acad Tobacco Sci, Guiyang 550081, Peoples R China

3.Tianjin Agr Univ, Coll Agron & Resources & Environm, Tianjin 300384, Peoples R China

4.Guizhou Acad Agr Sci, Guiyang 550006, Peoples R China

5.China Natl Tobacco Corp, Hainan Cigar Res Inst, Hainan Prov Branch, Haikou 571100, Hainan, Peoples R China

6.Zhejiang Univ, Coll Agr & Biotechnol, Dept Agron, Key Lab Crop Germplasm Resource, Hangzhou 310058, Peoples R China

关键词： Withering; Dehydration; Cell wall metabolism; Postharvest tobacco leaves; TMT-Based quantitative proteomics

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN： 0981-9428

年卷期： 2020 年 150 卷

页码：

收录情况： SCI

摘要： Withering is an important biological process accompanied by dehydration and cell wall metabolism in post-harvest plant organs during curing/processing and storage. However, dynamics involved in cell wall metabolism and resultant water loss during withering in postharvest tobacco leaves is not well-documented. Here, tandem mass tag (TMT)-based quantitative proteomic analysis in postharvest tobacco leaves (cultivar K326) under different withering conditions was performed. In total, 11,556 proteins were detected, among which 496 differentially abundant proteins (DAPs) were identified. To elucidate the withering mechanism of tobacco leaves, 27 DAPs associated with cell wall metabolism were screened. In particular, pectin acetylesterases, glucan endo-1,3-beta-glucosidases, xyloglucan endotransglucosylase/hydrolase, alpha-xylosidase 1-like, probable galactinol-sucrose galactosyltransferases, endochitinase A, chitotriosidase-1-like and expansin were the key proteins responsible for the withering of postharvest tobacco leaves. These DAPs were mainly involved in pectin metabolism, cellulose, hemicellulose and galactose metabolism, amino sugar and nucleotide sugar metabolism as well as cell wall expansion. Furthermore, relative water content and softness values were significantly and positively correlated. Thus, dehydration and cell wall metabolism were crucial for tobacco leaf withering under different conditions. Nine candidate DAPs were confirmed by parallel reaction monitoring (PRM) technique. These results provide new insights into the withering mechanism underlying postharvest physiological regulatory networks in plants/crops.

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