Effect of substituting different concentrations of soybean lecithin and egg yolk in tris-based extender on goat semen cryopreservation
文献类型： 外文期刊
作者： Sun, Lingwei 1 ; Fan, Wenhua 1 ; Wu, Caifeng 1 ; Zhang, Shushan 1 ; Dai, Jianjun 1 ; Zhang, Defu 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Inst Anim Sci & Vet Med, Shanghai 201106, Peoples R China
2.Shanghai Municipal Key Lab Agri Genet & Breeding, Div Anim Genet Engn, Shanghai 201106, Peoples R China
关键词： Goat semen; Cryopreservation; Soybean lecithin; Egg yolk; Oxidative stress
期刊名称：CRYOBIOLOGY （ 影响因子：2.487； 五年影响因子：2.641 ）
ISSN： 0011-2240
年卷期： 2020 年 92 卷
页码：
收录情况： SCI
摘要： This study aimed to investigate the effects of different concentrations of soybean lecithin (SL; 0.5%, 1%, and 1.5%) and egg yolk (EY) in Tris-based extenders on the semen quality parameters of post-thawed goat semen. Sixteen ejaculates were collected from eight healthy, mature Chongming White goats (3-5 years of age). Each ejaculate was divided into five equal aliquots, and then each pellet was diluted with one of the five Tris-based extenders containing 20% EY, 0.5% SL, 1% SL, 2% SL, or 3% SL. The cooled diluted semen was loaded into 0.5 mL polyvinyl French straws and cryopreserved in liquid nitrogen. Frozen semen samples were thawed at 37 degrees C and assessed for sperm motility, viability, plasma acrosome integrity, membrane integrity, and mitochondria integrity, and the spermatozoa were assessed for reactive oxygen species (ROS), superoxide dismutase (SOD), and malondialdehyde (MDA). The semen extended in the 2.0% SL extract tended to have a higher sperm viability (57.44%), motility (52.14%), membrane integrity (45.31%), acrosome integrity (52.96%), and mitochondrial activity (50.21%) than the other SL-based extender concentrations (P < 0.05). The 2.0% SL treatment group was equivalent to the semen extended in 20% EY (P > 0.05). The extenders supplemented 20% EY or 2.0% SL significantly increased the SOD activity and decreased the ROS and MDA activities compared to the other groups (P < 0.05). In conclusion, the extenders supplemented with 20% EY and 2.0% SL had similar effects on spermatozoa preservation. These results indicate that a soybean lecithin-based diluent may be used as an alternative extender to egg yolk for the cryopreservation of goat semen.
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