Bioinspired design of an artificial peroxidase: introducing key residues of native peroxidases into F43Y myoglobin with a Tyr-heme cross-link
文献类型： 外文期刊
作者： Liao, Fei 1 ; Xu, Jia-Kun 2 ; Luo, Jie 1 ; Gao, Shu-Qin 3 ; Wang, Xiao-Juan 2 ; Lin, Ying-Wu 1 ;
作者机构： 1.Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China
2.Chinese Acad Fishery Sci, Minist Agr & Rural Affairs, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fisheries,Lab Marin, Qingdao 266071, Peoples R China
3.Univ South China, Lab Prot Struct & Funct, Hengyang 421001, Peoples R China
期刊名称：DALTON TRANSACTIONS （ 影响因子：4.39； 五年影响因子：4.07 ）
ISSN： 1477-9226
年卷期： 2020 年 49 卷 16 期
页码：
收录情况： SCI
摘要： Inspired by the structural features of native peroxidases, an artificial peroxidase was rationally designed using F43Y myoglobin with a Tyr-heme cross-link by further introduction of key residues, including both a distal Arg and a Trp close to the heme group, which exhibits an enhanced peroxidase activity similar to the most efficient native horseradish peroxidase. This study provides a simple approach for design of artificial heme enzymes by the combination of catalytic elements of native enzymes with the post-translational modifications of heme proteins.
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